Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2321883..2322472 Replicon chromosome
Accession NZ_CP128519
Organism Mannheimia haemolytica strain 192036

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag QTV54_RS12235 Protein ID WP_006248516.1
Coordinates 2322140..2322472 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag QTV54_RS12230 Protein ID WP_006248517.1
Coordinates 2321883..2322143 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTV54_RS12205 2317062..2318207 - 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -
QTV54_RS12210 2318819..2319463 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
QTV54_RS12215 2319465..2320253 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
QTV54_RS12220 2320261..2321058 + 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
QTV54_RS12225 2321145..2321813 + 669 WP_006248518.1 phosphoglycolate phosphatase -
QTV54_RS12230 2321883..2322143 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
QTV54_RS12235 2322140..2322472 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QTV54_RS12240 2322599..2323630 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
QTV54_RS12245 2323694..2324449 - 756 WP_006248513.1 M48 family metallopeptidase -
QTV54_RS12250 2324505..2325032 - 528 WP_006248512.1 inorganic diphosphatase -
QTV54_RS12270 2325612..2326646 + 1035 WP_006248509.1 endolytic transglycosylase MltG -
QTV54_RS12275 2326657..2327277 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T284734 WP_006248516.1 NZ_CP128519:2322140-2322472 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT284734 WP_006248517.1 NZ_CP128519:2321883-2322143 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References