Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2313042..2313631 Replicon chromosome
Accession NZ_CP128516
Organism Mannheimia haemolytica strain 192916

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag QTV68_RS12170 Protein ID WP_006248516.1
Coordinates 2313299..2313631 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag QTV68_RS12165 Protein ID WP_006248517.1
Coordinates 2313042..2313302 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTV68_RS12140 2308221..2309366 - 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -
QTV68_RS12145 2309978..2310622 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
QTV68_RS12150 2310624..2311412 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
QTV68_RS12155 2311420..2312217 + 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
QTV68_RS12160 2312304..2312972 + 669 WP_006248518.1 phosphoglycolate phosphatase -
QTV68_RS12165 2313042..2313302 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
QTV68_RS12170 2313299..2313631 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QTV68_RS12175 2313758..2314789 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
QTV68_RS12180 2314853..2315608 - 756 WP_006248513.1 M48 family metallopeptidase -
QTV68_RS12185 2315664..2316191 - 528 WP_006248512.1 inorganic diphosphatase -
QTV68_RS12205 2316771..2317805 + 1035 WP_006248509.1 endolytic transglycosylase MltG -
QTV68_RS12210 2317816..2318436 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T284716 WP_006248516.1 NZ_CP128516:2313299-2313631 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT284716 WP_006248517.1 NZ_CP128516:2313042-2313302 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References