Detailed information of TA system TA028469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-UPF0150
• Location: 686719..687125
• Replicon: chromosome
• Accession: NC_019427
• Organism: Anabaena sp. 90

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: K7WSH9
• Locus tag: ANA_RS03135
• Protein ID: WP_015078600.1
• Coordinates: 686719..686901 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ANA_RS03140
• Protein ID: WP_015078601.1
• Coordinates: 686898..687125 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANA_RS03100	682122..682724	+	603	WP_015078592.1	cell division protein SepF	-
ANA_RS03105	682782..683597	+	816	WP_015078593.1	pyrroline-5-carboxylate reductase	-
ANA_RS03110	683685..684056	-	372	WP_015078594.1	DUF5615 family PIN-like protein	-
ANA_RS03115	684053..684283	-	231	WP_015078595.1	DUF433 domain-containing protein	-
ANA_RS03120	684318..684524	-	207	WP_015078596.1	DUF2281 domain-containing protein	-
ANA_RS03125	684669..684920	-	252	WP_015078598.1	hypothetical protein	-
ANA_RS03130	685129..686358	-	1230	WP_015078599.1	GTPase domain-containing protein	-
ANA_RS03135	686719..686901	+	183	WP_015078600.1	type II toxin-antitoxin system HicA family toxin	Toxin
ANA_RS03140	686898..687125	+	228	WP_015078601.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ANA_RS03145	687153..687761	+	609	WP_015078602.1	hypothetical protein	-
ANA_RS03150	687799..688233	+	435	WP_015078603.1	DUF29 domain-containing protein	-
ANA_RS03155	688394..691075	+	2682	WP_015078604.1	RNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-66)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6679.88 Da
• Isoelectric Point: 10.6223

>T28469 WP_015078600.1 NC_019427:686719-686901 [Anabaena sp. 90]
MKVKEVLKILEADGWYIDRTRGSHRILKHPSKSGIVVVPGKPSDDIPVGTLSSIWKQAKL

Nucleotide

• Length: 183 bp

>T28469 NC_019427:686719-686901 [Anabaena sp. 90]
ATGAAGGTTAAAGAAGTTCTAAAAATTCTGGAAGCAGATGGTTGGTATATAGATCGTACCAGAGGTAGTCATCGTATCCT
CAAACATCCAAGCAAATCAGGTATTGTAGTTGTACCCGGAAAGCCAAGTGATGATATTCCAGTCGGTACACTCTCATCAA
TTTGGAAGCAAGCAAAATTATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8268.41 Da
• Isoelectric Point: 3.8954

>AT28469 WP_015078601.1 NC_019427:686898-687125 [Anabaena sp. 90]
MNEKMIEYTVIYERGQTNWGAYVPDLPGCVSIGDTLAEVQENIKEAIALYLEVLKEDGQPIPEPSTEVGKVAVTI

Nucleotide

• Length: 228 bp

>AT28469 NC_019427:686898-687125 [Anabaena sp. 90]
ATGAACGAAAAAATGATTGAGTACACAGTCATCTACGAACGTGGCCAGACAAACTGGGGCGCTTATGTTCCCGACTTACC
TGGTTGTGTCAGCATTGGCGATACTTTAGCAGAAGTACAGGAAAATATTAAAGAAGCGATCGCATTATACCTAGAAGTAT
TGAAAGAAGATGGACAACCCATACCCGAACCATCTACAGAAGTTGGTAAAGTTGCAGTGACGATATAA

Structures

Toxin

• AlphaFold DB: K7WSH9