Detailed information of TA system TA028467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3503686..3504247
• Replicon: chromosome
• Accession: NC_019395
• Organism: Propionibacterium acidipropionici ATCC 4875

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K7S927
• Locus tag: PACID_RS16085
• Protein ID: WP_015071954.1
• Coordinates: 3503686..3504033 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K7S105
• Locus tag: PACID_RS16090
• Protein ID: WP_015071955.1
• Coordinates: 3504020..3504247 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PACID_RS18670	3499245..3499796	+	552	WP_015071949.1	zinc-binding dehydrogenase	-
PACID_RS18010	3499953..3500579	-	627	WP_095532357.1	L,D-transpeptidase	-
PACID_RS16075	3500860..3502407	-	1548	WP_015071952.1	glycoside hydrolase family 32 protein	-
PACID_RS16080	3502574..3503533	+	960	WP_015071953.1	PfkB domain-containing protein	-
PACID_RS16085	3503686..3504033	-	348	WP_015071954.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PACID_RS16090	3504020..3504247	-	228	WP_015071955.1	ribbon-helix-helix domain-containing protein	Antitoxin
PACID_RS16095	3504391..3504630	+	240	WP_015071956.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
PACID_RS16100	3504627..3505055	+	429	WP_015071957.1	PIN domain-containing protein	-
PACID_RS16105	3505071..3507221	+	2151	WP_015071958.1	NAD(+) synthase	-
PACID_RS16110	3507315..3507719	-	405	WP_015071959.1	nitrite reductase small subunit NirD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-35)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12548.53 Da
• Isoelectric Point: 11.4911

>T28467 WP_015071954.1 NC_019395:c3504033-3503686 [Acidipropionibacterium acidipropionici ATCC 4875]
MIRGEVRLVDLDPARPGEVNKRRPAVLVSNDTANRTAARLGRGVVTVVPVTSNVTRVFPFQVLLQADETGLRRDSKAQAE
QVRSVSVERIGPVLGMVPADLMSRIDDALRIHLGL

Nucleotide

• Length: 348 bp

>T28467 NC_019395:c3504033-3503686 [Acidipropionibacterium acidipropionici ATCC 4875]
ATGATCCGCGGTGAGGTCCGGCTGGTCGATCTGGATCCGGCGCGCCCCGGCGAGGTGAACAAGCGACGTCCCGCCGTCCT
CGTGAGCAATGACACGGCGAACCGGACGGCCGCACGGCTGGGGCGGGGAGTGGTGACGGTGGTGCCGGTCACCAGCAACG
TCACCCGGGTCTTCCCCTTCCAGGTCCTGCTTCAGGCGGACGAGACGGGGCTGCGGCGCGATTCCAAGGCCCAGGCCGAG
CAGGTCCGCTCGGTGTCGGTGGAACGGATCGGCCCGGTGCTCGGGATGGTGCCGGCTGATCTCATGAGCCGCATCGATGA
CGCGCTGAGAATCCACCTGGGGCTGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8161.01 Da
• Isoelectric Point: 4.0941

>AT28467 WP_015071955.1 NC_019395:c3504247-3504020 [Acidipropionibacterium acidipropionici ATCC 4875]
MKLSVSLSKEQVETIDRYIRATGMRSRSAVIQKAIGMLGAPELEGDYAAAWDEWESGDAEAWESTAADGLGDDPR

Nucleotide

• Length: 228 bp

>AT28467 NC_019395:c3504247-3504020 [Acidipropionibacterium acidipropionici ATCC 4875]
ATGAAACTCAGTGTCAGCCTGTCCAAGGAGCAGGTGGAGACGATCGACCGGTACATCCGTGCCACCGGGATGAGGTCGAG
GTCCGCGGTGATCCAGAAGGCGATCGGCATGCTCGGCGCCCCGGAGCTCGAGGGGGACTACGCCGCCGCCTGGGACGAGT
GGGAGTCCGGGGACGCCGAGGCCTGGGAGTCGACGGCCGCCGATGGGCTCGGCGATGATCCGCGGTGA

Structures

Toxin

• AlphaFold DB: K7S927

Antitoxin

• AlphaFold DB: K7S105