Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3513608..3514245 Replicon chromosome
Accession NZ_CP128504
Organism Bacillus velezensis strain SRCM123434

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QTN49_RS17495 Protein ID WP_003156187.1
Coordinates 3513608..3513958 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QTN49_RS17500 Protein ID WP_003156188.1
Coordinates 3513964..3514245 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTN49_RS17455 (3508648) 3508648..3509250 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
QTN49_RS17460 (3509250) 3509250..3510038 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QTN49_RS17465 (3510004) 3510004..3510486 - 483 WP_007609591.1 anti-sigma B factor RsbW -
QTN49_RS17470 (3510483) 3510483..3510812 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QTN49_RS17475 (3510876) 3510876..3511883 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
QTN49_RS17480 (3511895) 3511895..3512296 - 402 WP_003156178.1 anti-sigma regulatory factor -
QTN49_RS17485 (3512299) 3512299..3512664 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
QTN49_RS17490 (3512669) 3512669..3513490 - 822 WP_025649964.1 STAS domain-containing protein -
QTN49_RS17495 (3513608) 3513608..3513958 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QTN49_RS17500 (3513964) 3513964..3514245 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QTN49_RS17505 (3514365) 3514365..3515534 - 1170 WP_104843106.1 alanine racemase -
QTN49_RS17510 (3515651) 3515651..3516658 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QTN49_RS17515 (3516823) 3516823..3517188 - 366 WP_007609580.1 holo-ACP synthase -
QTN49_RS17520 (3517281) 3517281..3517880 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T284664 WP_003156187.1 NZ_CP128504:c3513958-3513608 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT284664 WP_003156188.1 NZ_CP128504:c3514245-3513964 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References