Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3762490..3763127 Replicon chromosome
Accession NZ_CP128503
Organism Bacillus velezensis strain SRCM123422

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QTN48_RS19410 Protein ID WP_003156187.1
Coordinates 3762490..3762840 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QTN48_RS19415 Protein ID WP_003156188.1
Coordinates 3762846..3763127 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTN48_RS19370 (3757530) 3757530..3758132 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
QTN48_RS19375 (3758132) 3758132..3758920 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QTN48_RS19380 (3758886) 3758886..3759368 - 483 WP_003156173.1 anti-sigma B factor RsbW -
QTN48_RS19385 (3759365) 3759365..3759694 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QTN48_RS19390 (3759758) 3759758..3760765 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
QTN48_RS19395 (3760777) 3760777..3761178 - 402 WP_003156178.1 anti-sigma regulatory factor -
QTN48_RS19400 (3761181) 3761181..3761546 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
QTN48_RS19405 (3761551) 3761551..3762372 - 822 WP_003156182.1 STAS domain-containing protein -
QTN48_RS19410 (3762490) 3762490..3762840 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QTN48_RS19415 (3762846) 3762846..3763127 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QTN48_RS19420 (3763247) 3763247..3764416 - 1170 WP_063636967.1 alanine racemase -
QTN48_RS19425 (3764533) 3764533..3765540 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QTN48_RS19430 (3765705) 3765705..3766070 - 366 WP_014304402.1 holo-ACP synthase -
QTN48_RS19435 (3766163) 3766163..3766762 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T284662 WP_003156187.1 NZ_CP128503:c3762840-3762490 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT284662 WP_003156188.1 NZ_CP128503:c3763127-3762846 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References