Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 337906..338543 Replicon chromosome
Accession NZ_CP128501
Organism Bacillus amyloliquefaciens strain SRCM123386

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QTN46_RS01665 Protein ID WP_003156187.1
Coordinates 338193..338543 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QTN46_RS01660 Protein ID WP_003156188.1
Coordinates 337906..338187 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTN46_RS01640 (334272) 334272..334871 - 600 WP_289394151.1 rhomboid family intramembrane serine protease -
QTN46_RS01645 (334964) 334964..335329 + 366 WP_289394152.1 holo-ACP synthase -
QTN46_RS01650 (335494) 335494..336501 + 1008 WP_289394153.1 outer membrane lipoprotein carrier protein LolA -
QTN46_RS01655 (336618) 336618..337787 + 1170 WP_289394154.1 alanine racemase -
QTN46_RS01660 (337906) 337906..338187 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QTN46_RS01665 (338193) 338193..338543 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QTN46_RS01670 (338664) 338664..339485 + 822 WP_289394155.1 STAS domain-containing protein -
QTN46_RS01675 (339490) 339490..339855 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
QTN46_RS01680 (339858) 339858..340259 + 402 WP_013351097.1 anti-sigma regulatory factor -
QTN46_RS01685 (340271) 340271..341278 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
QTN46_RS01690 (341342) 341342..341671 + 330 WP_014469786.1 anti-sigma factor antagonist -
QTN46_RS01695 (341668) 341668..342150 + 483 WP_013351100.1 anti-sigma B factor RsbW -
QTN46_RS01700 (342116) 342116..342904 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
QTN46_RS01705 (342904) 342904..343506 + 603 WP_013351102.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T284655 WP_003156187.1 NZ_CP128501:338193-338543 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT284655 WP_003156188.1 NZ_CP128501:337906-338187 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References