Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3358199..3358836 Replicon chromosome
Accession NZ_CP128500
Organism Bacillus amyloliquefaciens strain SRCM123364

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QTN45_RS17330 Protein ID WP_003156187.1
Coordinates 3358199..3358549 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QTN45_RS17335 Protein ID WP_003156188.1
Coordinates 3358555..3358836 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTN45_RS17290 (QTN45_17290) 3353236..3353838 - 603 WP_013351102.1 PP2C family serine/threonine-protein phosphatase -
QTN45_RS17295 (QTN45_17295) 3353838..3354626 - 789 WP_013351101.1 RNA polymerase sigma factor SigB -
QTN45_RS17300 (QTN45_17300) 3354592..3355074 - 483 WP_013351100.1 anti-sigma B factor RsbW -
QTN45_RS17305 (QTN45_17305) 3355071..3355400 - 330 WP_014469786.1 anti-sigma factor antagonist -
QTN45_RS17310 (QTN45_17310) 3355464..3356471 - 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
QTN45_RS17315 (QTN45_17315) 3356483..3356884 - 402 WP_013351097.1 anti-sigma regulatory factor -
QTN45_RS17320 (QTN45_17320) 3356887..3357252 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
QTN45_RS17325 (QTN45_17325) 3357257..3358078 - 822 WP_013351096.1 STAS domain-containing protein -
QTN45_RS17330 (QTN45_17330) 3358199..3358549 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QTN45_RS17335 (QTN45_17335) 3358555..3358836 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QTN45_RS17340 (QTN45_17340) 3358955..3360124 - 1170 WP_013351095.1 alanine racemase -
QTN45_RS17345 (QTN45_17345) 3360241..3361248 - 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
QTN45_RS17350 (QTN45_17350) 3361413..3361778 - 366 WP_013351093.1 holo-ACP synthase -
QTN45_RS17355 (QTN45_17355) 3361871..3362470 + 600 WP_013351092.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T284654 WP_003156187.1 NZ_CP128500:c3358549-3358199 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT284654 WP_003156188.1 NZ_CP128500:c3358836-3358555 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References