Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 12355..13492 Replicon plasmid pRE25
Accession NZ_CP128463
Organism Enterococcus faecalis strain RE25

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag QSV41_RS00130 Protein ID WP_002332783.1
Coordinates 12355..13218 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag QSV41_RS00135 Protein ID WP_002326825.1
Coordinates 13220..13492 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QSV41_RS00100 (QSV41_00100) 7370..9364 - 1995 WP_229205849.1 MobA/MobL family protein -
QSV41_RS00105 (QSV41_00105) 9647..9946 + 300 WP_002332653.1 hypothetical protein -
QSV41_RS00110 (QSV41_00110) 9949..10206 + 258 WP_000002668.1 hypothetical protein -
QSV41_RS00115 (QSV41_00115) 10300..10533 - 234 WP_002332654.1 hypothetical protein -
QSV41_RS00120 (QSV41_00120) 10567..11064 - 498 WP_002338758.1 hypothetical protein -
QSV41_RS00125 (QSV41_00125) 11598..11915 - 318 WP_002326830.1 hypothetical protein -
QSV41_RS00130 (QSV41_00130) 12355..13218 - 864 WP_002332783.1 zeta toxin family protein Toxin
QSV41_RS00135 (QSV41_00135) 13220..13492 - 273 WP_002326825.1 antitoxin Antitoxin
QSV41_RS00140 (QSV41_00140) 13510..13725 - 216 WP_001835296.1 peptide-binding protein -
QSV41_RS00145 (QSV41_00145) 13817..14713 - 897 WP_002326827.1 ParA family protein -
QSV41_RS00150 (QSV41_00150) 14816..15076 - 261 Protein_19 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
QSV41_RS00155 (QSV41_00155) 15246..15983 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
QSV41_RS00160 (QSV41_00160) 16108..16191 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
QSV41_RS00165 (QSV41_00165) 16240..16479 - 240 WP_000635249.1 peptide-binding protein -
QSV41_RS00170 (QSV41_00170) 16613..17830 - 1218 Protein_23 DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / cat(pC221) / aph(3')-III / ant(6)-Ia - 1..50228 50228
- inside IScluster/Tn erm(B) / cat(pC221) / aph(3')-III / ant(6)-Ia - 15246..44068 28822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T284581 WP_002332783.1 NZ_CP128463:c13218-12355 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT284581 WP_002326825.1 NZ_CP128463:c13492-13220 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References