Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/-
Location 14749..15422 Replicon plasmid pPsv48A
Accession NC_019265
Organism Pseudomonas savastanoi

Toxin (Protein)


Gene name vapC Uniprot ID H1ZWW2
Locus tag PSA3335_RS00045 Protein ID WP_002556053.1
Coordinates 14749..15168 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag PSA3335_RS00050 Protein ID WP_003381346.1
Coordinates 15165..15422 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSA3335_RS00040 (PSA3335_A0007) 14076..14708 - 633 WP_015060648.1 recombinase family protein -
PSA3335_RS00045 (PSA3335_A0008) 14749..15168 - 420 WP_002556053.1 type II toxin-antitoxin system VapC family toxin Toxin
PSA3335_RS00050 (PSA3335_A0009) 15165..15422 - 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
PSA3335_RS00055 15575..15856 + 282 WP_060407302.1 hypothetical protein -
PSA3335_RS00060 (PSA3335_A0011) 15858..17054 + 1197 WP_237772646.1 IS91 family transposase -
PSA3335_RS00065 (PSA3335_A0013) 17851..19073 + 1223 WP_095178853.1 IS3-like element IS51 family transposase -
PSA3335_RS00070 (PSA3335_A0014) 19110..19796 - 687 WP_244657733.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..80123 80123


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15168.46 Da        Isoelectric Point: 4.7010

>T28443 WP_002556053.1 NC_019265:c15168-14749 [Pseudomonas savastanoi pv. savastanoi]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWET

Download         Length: 420 bp

>T28443 NC_019265:c15168-14749 [Pseudomonas savastanoi pv. savastanoi]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTAGAAACGCTTTATCTGTCCACCATCACCGTAGCCGAGTTGCGTGCAGGTATCGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAACGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT28443 WP_003381346.1 NC_019265:c15422-15165 [Pseudomonas savastanoi pv. savastanoi]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT28443 NC_019265:c15422-15165 [Pseudomonas savastanoi pv. savastanoi]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCCATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACATACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M2ZA27

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References