284371

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2060781..2061418 Replicon chromosome
Accession NZ_CP128184
Organism Bacillus velezensis strain YJ0-1 isolate soil

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QRA13_RS09875 Protein ID WP_003156187.1
Coordinates 2060781..2061131 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QRA13_RS09880 Protein ID WP_003156188.1
Coordinates 2061137..2061418 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRA13_RS09835 (QRA13_09835) 2055821..2056423 - 603 WP_082999005.1 PP2C family serine/threonine-protein phosphatase -
QRA13_RS09840 (QRA13_09840) 2056423..2057211 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QRA13_RS09845 (QRA13_09845) 2057177..2057659 - 483 WP_007609591.1 anti-sigma B factor RsbW -
QRA13_RS09850 (QRA13_09850) 2057656..2057985 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QRA13_RS09855 (QRA13_09855) 2058049..2059056 - 1008 WP_044802655.1 PP2C family protein-serine/threonine phosphatase -
QRA13_RS09860 (QRA13_09860) 2059068..2059469 - 402 WP_003156178.1 anti-sigma regulatory factor -
QRA13_RS09865 (QRA13_09865) 2059472..2059837 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
QRA13_RS09870 (QRA13_09870) 2059842..2060663 - 822 WP_003156182.1 STAS domain-containing protein -
QRA13_RS09875 (QRA13_09875) 2060781..2061131 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QRA13_RS09880 (QRA13_09880) 2061137..2061418 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QRA13_RS09885 (QRA13_09885) 2061538..2062707 - 1170 WP_038457028.1 alanine racemase -
QRA13_RS09890 (QRA13_09890) 2062824..2063831 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QRA13_RS09895 (QRA13_09895) 2063996..2064361 - 366 WP_007609580.1 holo-ACP synthase -
QRA13_RS09900 (QRA13_09900) 2064454..2065053 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T284371 WP_003156187.1 NZ_CP128184:c2061131-2060781 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT284371 WP_003156188.1 NZ_CP128184:c2061418-2061137 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References