Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5176196..5176772 Replicon chromosome
Accession NZ_CP127873
Organism Klebsiella michiganensis strain ELP1.34B

Toxin (Protein)


Gene name shpA Uniprot ID A0A1Q8YXR6
Locus tag QRD21_RS24140 Protein ID WP_046878034.1
Coordinates 5176485..5176772 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag QRD21_RS24135 Protein ID WP_025108476.1
Coordinates 5176196..5176498 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRD21_RS24120 (QRD21_24120) 5173035..5173370 + 336 WP_046878037.1 endoribonuclease SymE -
QRD21_RS24125 (QRD21_24125) 5173824..5174735 + 912 WP_286114901.1 acetamidase/formamidase family protein -
QRD21_RS24130 (QRD21_24130) 5174732..5176075 + 1344 WP_049101852.1 APC family permease -
QRD21_RS24135 (QRD21_24135) 5176196..5176498 - 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
QRD21_RS24140 (QRD21_24140) 5176485..5176772 - 288 WP_046878034.1 BrnT family toxin Toxin
QRD21_RS24145 (QRD21_24145) 5177032..5177475 - 444 WP_047724618.1 FosA family fosfomycin resistance glutathione transferase -
QRD21_RS24150 (QRD21_24150) 5177469..5178377 - 909 WP_224260657.1 LysR family transcriptional regulator -
QRD21_RS24155 (QRD21_24155) 5178465..5179247 + 783 WP_286114906.1 NAD(P)H-dependent oxidoreductase -
QRD21_RS24160 (QRD21_24160) 5179395..5179979 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
QRD21_RS24165 (QRD21_24165) 5179997..5180797 + 801 WP_046878031.1 winged helix-turn-helix domain-containing protein -
QRD21_RS24170 (QRD21_24170) 5180794..5181312 + 519 WP_004108661.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5165052..5176775 11723


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11210.69 Da        Isoelectric Point: 7.4687

>T284328 WP_046878034.1 NZ_CP127873:c5176772-5176485 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT284328 WP_025108476.1 NZ_CP127873:c5176498-5176196 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YXR6

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References