Detailed information of TA system TA028428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 30126..30703
• Replicon: plasmid pLM1-2cUG1
• Accession: NC_018889
• Organism: Listeria monocytogenes SLCC2372

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: LMOSLCC2372_RS00145
• Protein ID: WP_003728465.1
• Coordinates: 30126..30467 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: LMOSLCC2372_RS00150
• Protein ID: WP_003728464.1
• Coordinates: 30461..30703 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMOSLCC2372_RS00130	26605..27159	-	555	WP_003728468.1	recombinase family protein	-
LMOSLCC2372_RS00135	27439..27798	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
LMOSLCC2372_RS00140	27798..29933	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
LMOSLCC2372_RS00145	30126..30467	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LMOSLCC2372_RS00150	30461..30703	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LMOSLCC2372_RS00155	30764..31015	-	252	WP_003728463.1	hypothetical protein	-
LMOSLCC2372_RS00160	31146..31472	+	327	WP_003728462.1	hypothetical protein	-
LMOSLCC2372_RS00165	31472..32134	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
LMOSLCC2372_RS00170	32153..32530	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
LMOSLCC2372_RS00175	32689..33177	+	489	WP_003728520.1	hypothetical protein	-
LMOSLCC2372_RS00180	33299..33547	+	249	WP_003728519.1	hypothetical protein	-
LMOSLCC2372_RS00185	33548..33973	+	426	WP_003728518.1	hypothetical protein	-
LMOSLCC2372_RS00190	33977..34699	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
LMOSLCC2372_RS00195	34715..35431	+	717	WP_015055880.1	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50100	50100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T28428 WP_003728465.1 NC_018889:c30467-30126 [Listeria monocytogenes SLCC2372]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T28428 NC_018889:c30467-30126 [Listeria monocytogenes SLCC2372]
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTT

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT28428 WP_003728464.1 NC_018889:c30703-30461 [Listeria monocytogenes SLCC2372]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT28428 NC_018889:c30703-30461 [Listeria monocytogenes SLCC2372]
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTT

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5