Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5236321..5236946 Replicon chromosome
Accession NZ_CP127839
Organism Klebsiella pneumoniae RYC492

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QRF73_RS25335 Protein ID WP_002882817.1
Coordinates 5236321..5236704 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QRF73_RS25340 Protein ID WP_004150355.1
Coordinates 5236704..5236946 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF73_RS25320 (QRF73_25315) 5233687..5234589 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QRF73_RS25325 (QRF73_25320) 5234586..5235221 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QRF73_RS25330 (QRF73_25325) 5235218..5236147 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QRF73_RS25335 (QRF73_25330) 5236321..5236704 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QRF73_RS25340 (QRF73_25335) 5236704..5236946 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QRF73_RS25345 (QRF73_25340) 5237151..5238068 + 918 WP_004895173.1 alpha/beta hydrolase -
QRF73_RS25350 (QRF73_25345) 5238082..5239023 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QRF73_RS25355 (QRF73_25350) 5239068..5239505 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QRF73_RS25360 (QRF73_25355) 5239502..5240362 - 861 WP_004146232.1 virulence factor BrkB family protein -
QRF73_RS25365 (QRF73_25360) 5240356..5240955 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T284271 WP_002882817.1 NZ_CP127839:c5236704-5236321 [Klebsiella pneumoniae RYC492]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT284271 WP_004150355.1 NZ_CP127839:c5236946-5236704 [Klebsiella pneumoniae RYC492]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References