Detailed information of TA system TA028427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2414991..2415969
• Replicon: chromosome
• Accession: NC_018877
• Organism: Bacillus thuringiensis Bt407

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: M1Q940
• Locus tag: BTB_RS12355
• Protein ID: WP_000624982.1
• Coordinates: 2414991..2415728 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: BTB_RS12360
• Protein ID: WP_000237819.1
• Coordinates: 2415841..2415969 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTB_RS12335	2410633..2411415	+	783	WP_000355672.1	class I SAM-dependent methyltransferase	-
BTB_RS12340	2411573..2413258	-	1686	WP_003270568.1	alpha-keto acid decarboxylase family protein	-
BTB_RS12345	2413366..2413848	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
BTB_RS12350	2414015..2414752	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BTB_RS12355	2414991..2415728	+	738	WP_000624982.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BTB_RS12360	2415841..2415969	+	129	WP_000237819.1	hypothetical protein	Antitoxin
BTB_RS34865	2416042..2416218	+	177	WP_000852631.1	hypothetical protein	-
BTB_RS12370	2416262..2416537	-	276	WP_001032898.1	hypothetical protein	-
BTB_RS34870	2417048..2417224	+	177	WP_000852630.1	hypothetical protein	-
BTB_RS12380	2417368..2418837	+	1470	WP_000287547.1	beta-Ala-His dipeptidase	-
BTB_RS12385	2419083..2419892	+	810	WP_000678371.1	C39 family peptidase	-
BTB_RS12390	2419918..2420520	+	603	WP_000517270.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28323.03 Da
• Isoelectric Point: 8.2998

>T28427 WP_000624982.1 NC_018877:2414991-2415728 [Bacillus thuringiensis Bt407]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T28427 NC_018877:2414991-2415728 [Bacillus thuringiensis Bt407]
ATGATCTCTAACATTCGAATTGGCCTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT28427 WP_000237819.1 NC_018877:2415841-2415969 [Bacillus thuringiensis Bt407]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT28427 NC_018877:2415841-2415969 [Bacillus thuringiensis Bt407]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: M1Q940

Antitoxin

• AlphaFold DB: M1PKC2