Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4636712..4637522 Replicon chromosome
Accession NZ_CP127839
Organism Klebsiella pneumoniae RYC492

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag QRF73_RS22485 Protein ID WP_004178461.1
Coordinates 4636712..4637245 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag QRF73_RS22490 Protein ID WP_002887278.1
Coordinates 4637256..4637522 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF73_RS22480 (QRF73_22475) 4635543..4636664 + 1122 WP_002887282.1 cupin domain-containing protein -
QRF73_RS22485 (QRF73_22480) 4636712..4637245 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
QRF73_RS22490 (QRF73_22485) 4637256..4637522 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
QRF73_RS22495 (QRF73_22490) 4637625..4639058 - 1434 WP_004894565.1 Cu(+)/Ag(+) sensor histidine kinase -
QRF73_RS22500 (QRF73_22495) 4639048..4639731 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
QRF73_RS22505 (QRF73_22500) 4639903..4641288 + 1386 WP_004894567.1 efflux transporter outer membrane subunit -
QRF73_RS22510 (QRF73_22505) 4641306..4641650 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T284268 WP_004178461.1 NZ_CP127839:c4637245-4636712 [Klebsiella pneumoniae RYC492]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT284268 WP_002887278.1 NZ_CP127839:c4637522-4637256 [Klebsiella pneumoniae RYC492]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References