28426

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238836..239478 Replicon chromosome
Accession NC_018877
Organism Bacillus thuringiensis Bt407

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BTB_RS01335 Protein ID WP_000635965.1
Coordinates 239128..239478 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BTB_RS01330 Protein ID WP_000004570.1
Coordinates 238836..239123 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTB_RS01305 234153..235115 + 963 WP_000961144.1 UV DNA damage repair endonuclease UvsE -
BTB_RS01310 235108..235680 - 573 WP_000908526.1 rhomboid family intramembrane serine protease -
BTB_RS01315 235774..236133 + 360 WP_000583419.1 holo-ACP synthase -
BTB_RS01320 236290..237240 + 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
BTB_RS01325 237358..238527 + 1170 WP_000390593.1 alanine racemase -
BTB_RS01330 238836..239123 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BTB_RS01335 239128..239478 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTB_RS01340 239547..241715 + 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
BTB_RS32390 241773..241889 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
BTB_RS01345 242085..242543 + 459 WP_000503730.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T28426 WP_000635965.1 NC_018877:239128-239478 [Bacillus thuringiensis Bt407]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T28426 NC_018877:239128-239478 [Bacillus thuringiensis Bt407]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT28426 WP_000004570.1 NC_018877:238836-239123 [Bacillus thuringiensis Bt407]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT28426 NC_018877:238836-239123 [Bacillus thuringiensis Bt407]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References