Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 490685..491322 Replicon chromosome
Accession NZ_CP127834
Organism Bacillus velezensis strain Ag109

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NSY31_RS02485 Protein ID WP_003156187.1
Coordinates 490972..491322 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NSY31_RS02480 Protein ID WP_003156188.1
Coordinates 490685..490966 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NSY31_RS02460 (NSY31_02460) 487050..487649 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NSY31_RS02465 (NSY31_02465) 487742..488107 + 366 WP_007410229.1 holo-ACP synthase -
NSY31_RS02470 (NSY31_02470) 488272..489279 + 1008 WP_098081024.1 outer membrane lipoprotein carrier protein LolA -
NSY31_RS02475 (NSY31_02475) 489396..490565 + 1170 WP_098081026.1 alanine racemase -
NSY31_RS02480 (NSY31_02480) 490685..490966 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NSY31_RS02485 (NSY31_02485) 490972..491322 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NSY31_RS02490 (NSY31_02490) 491440..492261 + 822 WP_003156182.1 STAS domain-containing protein -
NSY31_RS02495 (NSY31_02495) 492266..492631 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NSY31_RS02500 (NSY31_02500) 492634..493035 + 402 WP_003156178.1 anti-sigma regulatory factor -
NSY31_RS02505 (NSY31_02505) 493047..494054 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
NSY31_RS02510 (NSY31_02510) 494118..494447 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NSY31_RS02515 (NSY31_02515) 494444..494926 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NSY31_RS02520 (NSY31_02520) 494892..495680 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NSY31_RS02525 (NSY31_02525) 495680..496282 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T284255 WP_003156187.1 NZ_CP127834:490972-491322 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp


Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT284255 WP_003156188.1 NZ_CP127834:490685-490966 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References