Detailed information of TA system TA028425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2108940..2109526
• Replicon: chromosome
• Accession: NC_018870
• Organism: Thermacetogenium phaeum DSM 12270

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K4LH27
• Locus tag: TPH_RS10325
• Protein ID: WP_015051151.1
• Coordinates: 2108940..2109287 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: TPH_RS10330
• Protein ID: WP_148275902.1
• Coordinates: 2109281..2109526 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TPH_RS10300	2104110..2104586	-	477	WP_015051146.1	CapA family protein	-
TPH_RS10305	2104640..2105053	-	414	Protein_2046	pyridoxal-phosphate dependent enzyme	-
TPH_RS10310	2105100..2106440	-	1341	WP_015051148.1	NTP transferase domain-containing protein	-
TPH_RS10315	2106440..2107594	-	1155	WP_015051149.1	inositol-3-phosphate synthase	-
TPH_RS10320	2107587..2108519	-	933	WP_015051150.1	histidinol-phosphatase HisJ family protein	-
TPH_RS10325	2108940..2109287	-	348	WP_015051151.1	endoribonuclease MazF	Toxin
TPH_RS10330	2109281..2109526	-	246	WP_148275902.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TPH_RS10335	2109979..2111022	-	1044	WP_015051153.1	hypothetical protein	-
TPH_RS10340	2111015..2112850	-	1836	WP_015051154.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-113)

Antitoxin

• Antitoxin PemI-like (5-81)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12648.78 Da
• Isoelectric Point: 8.4951

>T28425 WP_015051151.1 NC_018870:c2109287-2108940 [Thermacetogenium phaeum DSM 12270]
MVNEVKTYIPERGDLVWLQFDPQTGHEQAGRRPALVISPQLYNGKVGLALFCPVTTRAKGYPFEVEIPAGLKITGVILAD
QVKSLDWRARDAQFACKVPAGVMAEVQAKLQLLIS

Nucleotide

• Length: 348 bp

>T28425 NC_018870:c2109287-2108940 [Thermacetogenium phaeum DSM 12270]
ATGGTAAACGAGGTGAAAACCTATATTCCTGAACGTGGCGATCTTGTCTGGCTCCAGTTTGATCCTCAGACAGGACATGA
GCAGGCCGGAAGGCGGCCGGCGCTGGTAATTTCCCCGCAGCTTTATAACGGAAAGGTGGGGCTGGCCCTTTTCTGTCCTG
TTACTACAAGGGCGAAGGGTTATCCTTTTGAAGTAGAGATACCTGCCGGTTTAAAGATTACAGGAGTGATCCTGGCAGAC
CAGGTAAAAAGTCTTGACTGGCGGGCACGGGATGCACAATTTGCCTGCAAGGTCCCGGCAGGGGTGATGGCAGAGGTTCA
GGCTAAACTTCAGCTGTTGATCTCCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9042.46 Da
• Isoelectric Point: 7.2026

>AT28425 WP_148275902.1 NC_018870:c2109526-2109281 [Thermacetogenium phaeum DSM 12270]
VKIVMQPHVQKWGNSLGIRIPLSLAQKIGLKEGTPVDLEASDDAIIIRRKRYSLEQLLSQVTPDNLHGEVDTGTLVGREI
W

Nucleotide

• Length: 246 bp

>AT28425 NC_018870:c2109526-2109281 [Thermacetogenium phaeum DSM 12270]
GTGAAAATCGTCATGCAACCTCATGTGCAAAAGTGGGGCAATAGTTTGGGGATTCGCATCCCTTTATCGCTGGCACAAAA
AATTGGCCTTAAAGAAGGCACACCGGTTGATTTAGAGGCAAGTGATGACGCAATTATTATCCGCCGCAAACGTTATAGCC
TGGAACAATTGCTGTCCCAGGTTACACCTGATAATCTTCATGGCGAAGTAGATACAGGAACTCTGGTTGGGCGGGAAATA
TGGTAA

Structures

Toxin

• AlphaFold DB: K4LH27