Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 1661998..1662625 Replicon chromosome
Accession NC_018867
Organism Dehalobacter sp. CF

Toxin (Protein)


Gene name pemK Uniprot ID K4L4V2
Locus tag DCF50_RS07975 Protein ID WP_015043684.1
Coordinates 1661998..1662348 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1C2Y501
Locus tag DCF50_RS07980 Protein ID WP_019225279.1
Coordinates 1662338..1662625 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DCF50_RS07955 1657032..1657742 - 711 WP_015043680.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
DCF50_RS07960 1657815..1658240 - 426 WP_015043681.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
DCF50_RS07965 1658258..1659484 - 1227 WP_015043682.1 amidohydrolase -
DCF50_RS07970 1659687..1661825 - 2139 WP_015043683.1 RNA-binding transcriptional accessory protein -
DCF50_RS07975 1661998..1662348 - 351 WP_015043684.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DCF50_RS07980 1662338..1662625 - 288 WP_019225279.1 hypothetical protein Antitoxin
DCF50_RS07985 1662785..1663585 - 801 WP_015043686.1 glutamine amidotransferase -
DCF50_RS07990 1663582..1665009 - 1428 WP_015043687.1 Mur ligase family protein -
DCF50_RS07995 1665153..1665809 + 657 WP_015043688.1 gamma carbonic anhydrase family protein -
DCF50_RS08000 1665877..1666317 - 441 WP_015043689.1 MOSC domain-containing protein -
DCF50_RS08005 1666359..1666868 - 510 WP_015043690.1 molybdopterin-guanine dinucleotide biosynthesis protein B -
DCF50_RS08010 1666894..1667388 - 495 WP_015043691.1 MogA/MoaB family molybdenum cofactor biosynthesis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-38)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12801.78 Da        Isoelectric Point: 5.2375

>T28423 WP_015043684.1 NC_018867:c1662348-1661998 [Dehalobacter sp. CF]
MSIKRGEIFYAELNPVVGSEQGGTRPVLVIQNDIGNQYSPTTIIAAITSQISKAKLPTHVEVKAKRSGLERDSVILTEQI
RTIDKSRLKEKVAVLDEEVMLRVDEAIEISLGLTEI

Download         Length: 351 bp

>T28423 NC_018867:c1662348-1661998 [Dehalobacter sp. CF]
ATGAGTATTAAACGCGGGGAGATTTTTTATGCGGAACTAAACCCCGTTGTAGGTTCTGAACAAGGCGGCACCAGGCCGGT
CTTGGTTATTCAGAATGATATTGGAAACCAGTATAGCCCGACAACAATTATCGCAGCAATTACATCCCAGATTTCAAAAG
CCAAACTGCCGACCCATGTGGAAGTCAAAGCGAAACGAAGTGGTTTGGAAAGAGATTCTGTTATTTTGACTGAACAAATC
AGAACGATCGATAAGAGCCGTTTGAAGGAAAAAGTCGCAGTGCTCGACGAGGAAGTAATGCTCAGGGTTGATGAAGCTAT
TGAAATAAGCCTTGGGCTGACGGAGATCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10996.59 Da        Isoelectric Point: 4.3854

>AT28423 WP_019225279.1 NC_018867:c1662625-1662338 [Dehalobacter sp. CF]
MAETRRIVVCLPESIIEEVDEIVSSEKLNRSDFIKEAVYSVLIERRKAGIREQMRQGYVEMAQINLSMAVDLCQAEEEAT
MRYEGKLAWSVGYEY

Download         Length: 288 bp

>AT28423 NC_018867:c1662625-1662338 [Dehalobacter sp. CF]
TTGGCAGAGACTAGGCGTATTGTTGTATGTTTGCCGGAAAGCATTATTGAGGAAGTAGACGAAATCGTATCGTCAGAGAA
GTTAAACCGGAGTGATTTTATCAAAGAAGCTGTTTACAGTGTATTGATTGAACGGCGAAAAGCAGGAATACGAGAGCAGA
TGCGGCAGGGATATGTTGAAATGGCTCAAATCAACCTGTCGATGGCAGTAGATTTATGCCAGGCTGAAGAAGAGGCAACC
ATGCGTTATGAAGGGAAACTAGCTTGGAGTGTCGGGTATGAGTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K4L4V2

Antitoxin

Source ID Structure
AlphaFold DB A0A1C2Y501

References