Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 1634336..1634963 Replicon chromosome
Accession NC_018866
Organism Dehalobacter sp. DCA

Toxin (Protein)


Gene name pemK Uniprot ID K4L4V2
Locus tag DHBDCA_RS07870 Protein ID WP_015043684.1
Coordinates 1634336..1634686 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1C2Y501
Locus tag DHBDCA_RS07875 Protein ID WP_019225279.1
Coordinates 1634676..1634963 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHBDCA_RS07850 1629370..1630080 - 711 WP_015043680.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
DHBDCA_RS07855 1630153..1630578 - 426 WP_015043681.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
DHBDCA_RS07860 1630596..1631822 - 1227 WP_015043682.1 amidohydrolase -
DHBDCA_RS07865 1632025..1634163 - 2139 WP_015043683.1 RNA-binding transcriptional accessory protein -
DHBDCA_RS07870 1634336..1634686 - 351 WP_015043684.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DHBDCA_RS07875 1634676..1634963 - 288 WP_019225279.1 hypothetical protein Antitoxin
DHBDCA_RS07880 1635123..1635923 - 801 WP_015043686.1 glutamine amidotransferase -
DHBDCA_RS07885 1635920..1637347 - 1428 WP_015043687.1 Mur ligase family protein -
DHBDCA_RS07890 1637491..1638147 + 657 WP_015043688.1 gamma carbonic anhydrase family protein -
DHBDCA_RS07895 1638215..1638655 - 441 WP_015043689.1 MOSC domain-containing protein -
DHBDCA_RS07900 1638697..1639206 - 510 WP_015043690.1 molybdopterin-guanine dinucleotide biosynthesis protein B -
DHBDCA_RS07905 1639232..1639726 - 495 WP_015043691.1 MogA/MoaB family molybdenum cofactor biosynthesis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-38)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12801.78 Da        Isoelectric Point: 5.2375

>T28422 WP_015043684.1 NC_018866:c1634686-1634336 [Dehalobacter sp. DCA]
MSIKRGEIFYAELNPVVGSEQGGTRPVLVIQNDIGNQYSPTTIIAAITSQISKAKLPTHVEVKAKRSGLERDSVILTEQI
RTIDKSRLKEKVAVLDEEVMLRVDEAIEISLGLTEI

Download         Length: 351 bp

>T28422 NC_018866:c1634686-1634336 [Dehalobacter sp. DCA]
ATGAGTATTAAACGCGGGGAGATTTTTTATGCGGAACTAAACCCCGTTGTAGGTTCTGAACAAGGCGGCACCAGGCCGGT
CTTGGTTATTCAGAATGATATTGGAAACCAGTATAGCCCGACAACAATTATCGCAGCAATTACATCCCAGATTTCAAAAG
CCAAACTGCCGACCCATGTGGAAGTCAAAGCGAAACGAAGTGGTTTGGAAAGAGATTCTGTTATTTTGACTGAACAAATC
AGAACGATCGATAAGAGCCGTTTGAAGGAAAAAGTCGCAGTGCTCGACGAGGAAGTAATGCTCAGGGTTGATGAAGCTAT
TGAAATAAGCCTTGGGCTGACGGAGATCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10996.59 Da        Isoelectric Point: 4.3854

>AT28422 WP_019225279.1 NC_018866:c1634963-1634676 [Dehalobacter sp. DCA]
MAETRRIVVCLPESIIEEVDEIVSSEKLNRSDFIKEAVYSVLIERRKAGIREQMRQGYVEMAQINLSMAVDLCQAEEEAT
MRYEGKLAWSVGYEY

Download         Length: 288 bp

>AT28422 NC_018866:c1634963-1634676 [Dehalobacter sp. DCA]
TTGGCAGAGACTAGGCGTATTGTTGTATGTTTGCCGGAAAGCATTATTGAGGAAGTAGACGAAATCGTATCGTCAGAGAA
GTTAAACCGGAGTGATTTTATCAAAGAAGCTGTTTACAGTGTATTGATTGAACGGCGAAAAGCAGGAATACGAGAGCAGA
TGCGGCAGGGATATGTTGAAATGGCTCAAATCAACCTGTCGATGGCAGTAGATTTATGCCAGGCTGAAGAAGAGGCAACC
ATGCGTTATGAAGGGAAACTAGCTTGGAGTGTCGGGTATGAGTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K4L4V2

Antitoxin

Source ID Structure
AlphaFold DB A0A1C2Y501

References