Detailed information of TA system TA028420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3142916..3143447
• Replicon: chromosome
• Accession: NC_018829
• Organism: Bordetella bronchiseptica MO149

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: BN115_RS14645
• Protein ID: WP_003816744.1
• Coordinates: 3142916..3143182 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: BN115_RS23930
• Protein ID: WP_003816743.1
• Coordinates: 3143169..3143447 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN115_RS14625	3138151..3138861	-	711	WP_003816747.1	aquaporin Z	-
BN115_RS14630	3138969..3139601	-	633	WP_003812619.1	PAS domain-containing protein	-
BN115_RS14635	3139998..3140876	+	879	WP_003816745.1	tripartite tricarboxylate transporter substrate binding protein	-
BN115_RS14640	3140938..3142480	+	1543	Protein_2908	M81 family metallopeptidase	-
BN115_RS24070	3142608..3142798	+	191	Protein_2909	IS481 family transposase	-
BN115_RS14645	3142916..3143182	+	267	WP_003816744.1	BrnT family toxin	Toxin
BN115_RS23930	3143169..3143447	+	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
BN115_RS24145	3143476..3143652	+	177	WP_157938325.1	hypothetical protein	-
BN115_RS14655	3143803..3144459	-	657	WP_015041647.1	phosphoribosylanthranilate isomerase	-
BN115_RS14660	3144474..3146141	-	1668	WP_015041648.1	TRAP transporter large permease subunit	-
BN115_RS14665	3146144..3146773	-	630	WP_010930386.1	TRAP transporter small permease subunit	-
BN115_RS14670	3146985..3148079	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.44 Da
• Isoelectric Point: 9.4798

>T28420 WP_003816744.1 NC_018829:3142916-3143182 [Bordetella bronchiseptica MO149]
MDITYDPAKNDKNIADRGLSFELVRGFEWASALVVEDARQAYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T28420 NC_018829:3142916-3143182 [Bordetella bronchiseptica MO149]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGCGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATGCCCGCCAGGCCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT28420 WP_003816743.1 NC_018829:3143169-3143447 [Bordetella bronchiseptica MO149]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT28420 NC_018829:3143169-3143447 [Bordetella bronchiseptica MO149]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCAATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Antitoxin

• AlphaFold DB: K0MF02