Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4745745..4746321 Replicon chromosome
Accession NZ_CP127314
Organism Escherichia coli strain C25

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag QRM69_RS23005 Protein ID WP_001295743.1
Coordinates 4746034..4746321 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag QRM69_RS23000 Protein ID WP_000063148.1
Coordinates 4745745..4746047 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM69_RS22985 (4742341) 4742341..4744491 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QRM69_RS22990 (4744541) 4744541..4744744 + 204 WP_000467859.1 YbdD/YjiX family protein -
QRM69_RS22995 (4744755) 4744755..4745711 + 957 WP_001295745.1 GTPase -
QRM69_RS23000 (4745745) 4745745..4746047 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
QRM69_RS23005 (4746034) 4746034..4746321 - 288 WP_001295743.1 BrnT family toxin Toxin
QRM69_RS23010 (4746518) 4746518..4747684 + 1167 WP_000800831.1 restriction endonuclease -
QRM69_RS23015 (4747748) 4747748..4749367 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
QRM69_RS23020 (4749357) 4749357..4750661 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T284157 WP_001295743.1 NZ_CP127314:c4746321-4746034 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT284157 WP_000063148.1 NZ_CP127314:c4746047-4745745 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References