Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2997554..2999142 Replicon chromosome
Accession NZ_CP127314
Organism Escherichia coli strain C25

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag QRM69_RS14470 Protein ID WP_001125431.1
Coordinates 2997820..2999142 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag QRM69_RS14465 Protein ID WP_001304368.1
Coordinates 2997554..2997820 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM69_RS14450 (2993922) 2993922..2995373 + 1452 WP_000854660.1 tagaturonate reductase -
QRM69_RS14455 (2995570) 2995570..2996484 + 915 WP_001443133.1 bestrophin family protein -
QRM69_RS14460 (2996488) 2996488..2997246 - 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
QRM69_RS14465 (2997554) 2997554..2997820 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
QRM69_RS14470 (2997820) 2997820..2999142 + 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
QRM69_RS14475 (2999452) 2999452..2999859 - 408 Protein_2818 acetylxylan esterase -
QRM69_RS14480 (2999862) 2999862..3000443 - 582 Protein_2819 LysR family transcriptional regulator -
QRM69_RS14485 (3000592) 3000592..3001374 + 783 WP_001221834.1 SDR family oxidoreductase -
QRM69_RS14490 (3001618) 3001618..3001725 - 108 Protein_2821 hypothetical protein -
QRM69_RS14495 (3002150) 3002150..3002713 + 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
QRM69_RS14500 (3003075) 3003075..3003785 + 711 WP_001195181.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T284150 WP_001125431.1 NZ_CP127314:2997820-2999142 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT284150 WP_001304368.1 NZ_CP127314:2997554-2997820 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References