Detailed information of TA system TA028413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 2207205..2207736
• Replicon: chromosome
• Accession: NC_018828
• Organism: Bordetella parapertussis Bpp5

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: BN117_RS10445
• Protein ID: WP_003812626.1
• Coordinates: 2207470..2207736 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: BN117_RS10440
• Protein ID: WP_003816743.1
• Coordinates: 2207205..2207483 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN117_RS10415	2202636..2203730	+	1095	WP_015039627.1	TRAP transporter substrate-binding protein	-
BN117_RS10420	2203942..2204571	+	630	WP_003812635.1	TRAP transporter small permease subunit	-
BN117_RS10425	2204574..2206241	+	1668	WP_015039628.1	TRAP transporter large permease subunit	-
BN117_RS10430	2206256..2206912	+	657	WP_015039629.1	phosphoribosylanthranilate isomerase	-
BN117_RS10440	2207205..2207483	-	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
BN117_RS10445	2207470..2207736	-	267	WP_003812626.1	BrnT family toxin	Toxin
BN117_RS10450	2207967..2209508	-	1542	WP_015039630.1	M81 family metallopeptidase	-
BN117_RS10455	2209570..2210448	-	879	WP_015039631.1	tripartite tricarboxylate transporter substrate binding protein	-
BN117_RS10460	2210845..2211477	+	633	WP_003812619.1	PAS domain-containing protein	-
BN117_RS10465	2211588..2212298	+	711	WP_003812617.1	aquaporin Z	-
BN117_RS10470	2212337..2212609	-	273	WP_003812615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T28413 WP_003812626.1 NC_018828:c2207736-2207470 [Bordetella parapertussis Bpp5]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T28413 NC_018828:c2207736-2207470 [Bordetella parapertussis Bpp5]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT28413 WP_003816743.1 NC_018828:c2207483-2207205 [Bordetella parapertussis Bpp5]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT28413 NC_018828:c2207483-2207205 [Bordetella parapertussis Bpp5]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02