Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2544905..2545488 Replicon chromosome
Accession NZ_CP127306
Organism Escherichia coli strain C51

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag QRM70_RS12350 Protein ID WP_285965471.1
Coordinates 2545153..2545488 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QRM70_RS12345 Protein ID WP_000581937.1
Coordinates 2544905..2545153 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM70_RS12335 (2541244) 2541244..2542545 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QRM70_RS12340 (2542593) 2542593..2544827 + 2235 WP_000226815.1 GTP pyrophosphokinase -
QRM70_RS12345 (2544905) 2544905..2545153 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QRM70_RS12350 (2545153) 2545153..2545488 + 336 WP_285965471.1 endoribonuclease MazF Toxin
QRM70_RS12355 (2545559) 2545559..2546350 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
QRM70_RS12360 (2546578) 2546578..2548215 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QRM70_RS12365 (2548303) 2548303..2549601 + 1299 WP_000036723.1 phosphopyruvate hydratase -
QRM70_RS12370 (2549657) 2549657..2550019 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12214.18 Da        Isoelectric Point: 8.4777

>T284117 WP_285965471.1 NZ_CP127306:2545153-2545488 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPVVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVVLADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT284117 WP_000581937.1 NZ_CP127306:2544905-2545153 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References