Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1749801..1750601 Replicon chromosome
Accession NZ_CP127306
Organism Escherichia coli strain C51

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag QRM70_RS08400 Protein ID WP_000342449.1
Coordinates 1750074..1750601 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag QRM70_RS08395 Protein ID WP_001277108.1
Coordinates 1749801..1750067 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM70_RS08375 (1745459) 1745459..1746127 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
QRM70_RS08380 (1746120) 1746120..1747178 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
QRM70_RS08385 (1747423) 1747423..1748277 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
QRM70_RS08390 (1748548) 1748548..1749651 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
QRM70_RS08395 (1749801) 1749801..1750067 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
QRM70_RS08400 (1750074) 1750074..1750601 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
QRM70_RS08405 (1750598) 1750598..1750981 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
QRM70_RS08410 (1751405) 1751405..1752513 + 1109 Protein_1609 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
QRM70_RS08415 (1752561) 1752561..1753487 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
QRM70_RS08420 (1753484) 1753484..1754761 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
QRM70_RS08425 (1754758) 1754758..1755525 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T284112 WP_000342449.1 NZ_CP127306:1750074-1750601 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT284112 WP_001277108.1 NZ_CP127306:1749801-1750067 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References