Detailed information of TA system TA028411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpB-mazE/PRK09812-ChpS
• Location: 48990..49603
• Replicon: plasmid pND6-2
• Accession: NC_018746
• Organism: Pseudomonas putida ND6

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: I3V5I0
• Locus tag: YSA_RS29415
• Protein ID: WP_015026476.1
• Coordinates: 49235..49603 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: YSA_RS29410
• Protein ID: WP_041167414.1
• Coordinates: 48990..49238 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YSA_RS29375	44013..46088	+	2076	WP_015026468.1	DNA cytosine methyltransferase	-
YSA_RS29380	46078..46308	+	231	WP_015026469.1	hypothetical protein	-
YSA_RS29385	46305..46841	+	537	WP_015026470.1	hypothetical protein	-
YSA_RS29390	46838..47521	+	684	WP_015026471.1	hypothetical protein	-
YSA_RS29395	47530..47949	+	420	WP_015026472.1	hypothetical protein	-
YSA_RS29400	47978..48454	+	477	WP_015026473.1	single-stranded DNA-binding protein	-
YSA_RS29410	48990..49238	+	249	WP_041167414.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
YSA_RS29415	49235..49603	+	369	WP_015026476.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
YSA_RS29420	49600..49830	+	231	WP_015026477.1	hypothetical protein	-
YSA_RS29425	49997..50425	+	429	WP_015026478.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
YSA_RS29430	50415..51698	+	1284	WP_015026479.1	Y-family DNA polymerase	-
YSA_RS29435	51740..52192	-	453	WP_015026480.1	hypothetical protein	-
YSA_RS29440	52429..52923	-	495	WP_110389096.1	hypothetical protein	-
YSA_RS29445	52905..54251	-	1347	WP_009684314.1	replicative DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..117003	117003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 15783.43 Da
• Isoelectric Point: 12.8863

>T28411 WP_015026476.1 NC_018746:49235-49603 [Pseudomonas putida ND6]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 369 bp

>T28411 NC_018746:49235-49603 [Pseudomonas putida ND6]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 10656.47 Da
• Isoelectric Point: 12.7138

>AT28411 WP_041167414.1 NC_018746:48990-49238 [Pseudomonas putida ND6]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKK

Nucleotide

• Length: 249 bp

>AT28411 NC_018746:48990-49238 [Pseudomonas putida ND6]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3V5I0