Detailed information of TA system TA028408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 347948..348566
• Replicon: chromosome
• Accession: NC_018704
• Organism: Amphibacillus xylanus NBRC 15112

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K0IVJ7
• Locus tag: AXY_RS01845
• Protein ID: WP_015009085.1
• Coordinates: 348216..348566 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AXY_RS01840
• Protein ID: WP_015009084.1
• Coordinates: 347948..348211 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXY_RS01820	343562..343918	+	357	WP_015009080.1	holo-ACP synthase	-
AXY_RS01825	343987..345489	+	1503	WP_015009081.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
AXY_RS01830	345544..346566	+	1023	WP_015009082.1	outer membrane lipoprotein carrier protein LolA	-
AXY_RS01835	346677..347813	+	1137	WP_015009083.1	alanine racemase	-
AXY_RS01840	347948..348211	+	264	WP_015009084.1	hypothetical protein	Antitoxin
AXY_RS01845	348216..348566	+	351	WP_015009085.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AXY_RS01850	348751..349620	+	870	WP_015009086.1	RsbT co-antagonist protein RsbRA	-
AXY_RS01855	349625..349981	+	357	WP_015009087.1	STAS domain-containing protein	-
AXY_RS01860	349984..350382	+	399	WP_015009088.1	anti-sigma regulatory factor	-
AXY_RS01865	350400..351410	+	1011	WP_015009089.1	PP2C family protein-serine/threonine phosphatase	-
AXY_RS01870	351469..351807	+	339	WP_015009090.1	STAS domain-containing protein	-
AXY_RS01875	351804..352277	+	474	WP_015009091.1	anti-sigma B factor RsbW	-
AXY_RS01880	352252..353040	+	789	WP_015009092.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12913.98 Da
• Isoelectric Point: 5.7185

>T28408 WP_015009085.1 NC_018704:348216-348566 [Amphibacillus xylanus NBRC 15112]
MIVKRGEVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTVIVAAITAQIQKAKLPTHVEIEAEKYGFERDSVILLEQI
RTIDKQRLTDKITQLDGQMMDKIDQALEISLGLTHI

Nucleotide

• Length: 351 bp

>T28408 NC_018704:348216-348566 [Amphibacillus xylanus NBRC 15112]
TTGATCGTAAAAAGAGGCGAAGTTTATTTCGCTGACCTGTCACCAGTTGTTGGTTCTGAGCAAGGTGGCGTTCGTCCGGT
ACTAGTGATTCAAAATAACATCGGCAATCGATTTAGCCCGACCGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAATTGCCAACGCATGTTGAAATTGAAGCGGAAAAATACGGATTCGAACGTGATTCGGTAATTTTGCTTGAACAAATC
CGAACTATAGATAAACAGCGATTGACTGATAAAATCACGCAGTTAGATGGTCAGATGATGGATAAAATTGATCAAGCACT
TGAAATAAGTTTAGGTTTAACTCATATTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10007.56 Da
• Isoelectric Point: 4.3846

>AT28408 WP_015009084.1 NC_018704:347948-348211 [Amphibacillus xylanus NBRC 15112]
MKEVVVKLPENLLKEVNGLLVEEDKELSDLLIQAAKSYVNDKKEQHIREYMQRGYMEMAKINLNIAAESFLVEEEAEITL
ERLVSGV

Nucleotide

• Length: 264 bp

>AT28408 NC_018704:347948-348211 [Amphibacillus xylanus NBRC 15112]
ATGAAAGAAGTTGTTGTAAAGTTACCTGAAAACCTATTAAAAGAGGTGAATGGACTCTTGGTAGAAGAAGATAAAGAATT
AAGTGATCTGCTGATCCAAGCGGCAAAATCTTATGTAAATGATAAAAAAGAACAACACATACGAGAATATATGCAACGCG
GATATATGGAAATGGCAAAGATTAACCTTAACATCGCTGCAGAATCTTTTTTAGTTGAAGAGGAGGCCGAAATCACCTTA
GAACGCTTAGTGAGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: K0IVJ7