Detailed information of TA system TA028407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 190651..191449
• Replicon: chromosome
• Accession: NC_018704
• Organism: Amphibacillus xylanus NBRC 15112

Toxin (Protein)

• Gene name: -
• Uniprot ID: K0IV68
• Locus tag: AXY_RS01015
• Protein ID: WP_015008920.1
• Coordinates: 190651..191124 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: AXY_RS01020
• Protein ID: WP_015008921.1
• Coordinates: 191129..191449 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXY_RS01010	189410..190570	-	1161	WP_015008919.1	site-specific integrase	-
AXY_RS01015	190651..191124	-	474	WP_015008920.1	ImmA/IrrE family metallo-endopeptidase	Toxin
AXY_RS01020	191129..191449	-	321	WP_015008921.1	helix-turn-helix domain-containing protein	Antitoxin
AXY_RS01025	191981..192310	-	330	WP_041450062.1	hypothetical protein	-
AXY_RS01030	192374..192556	+	183	WP_015008923.1	helix-turn-helix domain-containing protein	-
AXY_RS01035	192570..192764	+	195	WP_015008924.1	hypothetical protein	-
AXY_RS01040	192826..193152	+	327	WP_015008925.1	hypothetical protein	-
AXY_RS01045	193244..194041	+	798	WP_015008926.1	hypothetical protein	-
AXY_RS01050	194042..194302	+	261	WP_015008927.1	hypothetical protein	-
AXY_RS12700	194310..194471	+	162	WP_015008928.1	hypothetical protein	-
AXY_RS01055	194458..195210	+	753	WP_015008929.1	phage antirepressor KilAC domain-containing protein	-
AXY_RS12160	195284..196333	+	1050	WP_015008930.1	DnaD domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	189410..237715	48305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (10-122)

Antitoxin

• Cro/C1-type helix-turn-helix domain (6-64)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 18512.33 Da
• Isoelectric Point: 7.7773

>T28407 WP_015008920.1 NC_018704:c191124-190651 [Amphibacillus xylanus NBRC 15112]
MQYEKLLIEADQHGVDIYEKPMQKRNKGLYCDKVIWINKYINTSTEKACVLAEELGHYHTSAGNILDQKNLNNIKQEKQA
RTWAYEKLVPLKKIIKAHKDGPKNKYELAEYLGVTEDFLEKAIERYIEKFGLSVKYKDYVICFEPLGVIEWFDNKNF

Nucleotide

• Length: 474 bp

>T28407 NC_018704:c191124-190651 [Amphibacillus xylanus NBRC 15112]
ATGCAATATGAAAAACTTCTTATAGAGGCAGATCAACATGGAGTAGACATCTATGAAAAGCCAATGCAGAAGAGAAATAA
AGGTTTATACTGTGACAAAGTTATTTGGATAAATAAATACATAAATACATCCACAGAAAAAGCCTGTGTTCTAGCTGAAG
AACTTGGCCATTATCATACATCAGCAGGTAACATACTTGATCAAAAAAATTTAAACAACATAAAACAAGAAAAGCAAGCT
CGTACATGGGCATATGAAAAATTAGTTCCATTAAAGAAAATTATTAAAGCACATAAAGATGGTCCAAAAAATAAGTATGA
GCTTGCAGAATATTTAGGAGTAACTGAAGATTTTCTAGAAAAAGCGATTGAACGTTATATTGAGAAGTTTGGTTTATCTG
TTAAGTATAAAGACTATGTAATCTGCTTTGAACCACTTGGTGTAATTGAGTGGTTTGATAATAAAAATTTTTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12616.10 Da
• Isoelectric Point: 4.7484

>AT28407 WP_015008921.1 NC_018704:c191449-191129 [Amphibacillus xylanus NBRC 15112]
MTAFDRVKKLCEEQKISIVELEEKVGFGRNSLYSWKKNNPSSEKLEKVADFFNVSTDYLLGRTDIKYIHEPDTIAAHHDG
EDWTEEELEEIERFKEFVRMKRSQQE

Nucleotide

• Length: 321 bp

>AT28407 NC_018704:c191449-191129 [Amphibacillus xylanus NBRC 15112]
ATGACTGCTTTTGATAGAGTAAAAAAATTATGTGAGGAACAAAAAATTTCTATTGTGGAACTAGAGGAAAAGGTTGGATT
TGGTAGAAACTCTTTATACTCTTGGAAGAAAAACAATCCTTCTTCTGAGAAATTAGAAAAAGTAGCAGACTTTTTCAACG
TCTCCACCGATTATCTATTAGGTCGTACAGATATAAAATACATACATGAGCCAGACACAATCGCAGCTCATCACGATGGA
GAGGACTGGACAGAAGAAGAATTAGAAGAAATAGAACGATTTAAAGAATTTGTAAGAATGAAGAGAAGCCAGCAGGAGTG
A

Structures

Toxin

• AlphaFold DB: K0IV68