Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4025522..4026359 Replicon chromosome
Accession NZ_CP127297
Organism Escherichia coli strain APEC20/16

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag QRM67_RS19840 Protein ID WP_000227784.1
Coordinates 4025817..4026359 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag QRM67_RS19835 Protein ID WP_001297137.1
Coordinates 4025522..4025833 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM67_RS19810 (4020542) 4020542..4021489 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
QRM67_RS19815 (4021511) 4021511..4023502 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
QRM67_RS19820 (4023492) 4023492..4024106 + 615 WP_286004879.1 cytochrome o ubiquinol oxidase subunit III -
QRM67_RS19825 (4024106) 4024106..4024435 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
QRM67_RS19830 (4024447) 4024447..4025337 + 891 WP_000971336.1 heme o synthase -
QRM67_RS19835 (4025522) 4025522..4025833 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
QRM67_RS19840 (4025817) 4025817..4026359 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
QRM67_RS19845 (4026415) 4026415..4027350 - 936 WP_001297127.1 tetratricopeptide repeat protein -
QRM67_RS19850 (4027758) 4027758..4029122 + 1365 WP_001000978.1 MFS transporter -
QRM67_RS19855 (4029250) 4029250..4029741 - 492 WP_001138904.1 nucleotide binding protein YajQ -
QRM67_RS19860 (4029909) 4029909..4030820 + 912 WP_000705849.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T284067 WP_000227784.1 NZ_CP127297:4025817-4026359 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT284067 WP_001297137.1 NZ_CP127297:4025522-4025833 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References