Detailed information of TA system TA028406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1651928..1652462
• Replicon: plasmid pSYMB
• Accession: NC_018701
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q92X99
• Locus tag: BN406_RS33815
• Protein ID: WP_010974986.1
• Coordinates: 1651928..1652224 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: BN406_RS33820
• Protein ID: WP_003527850.1
• Coordinates: 1652214..1652462 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS33790	1647370..1648773	-	1404	WP_026029937.1	sodium:alanine symporter family protein	-
BN406_RS33795	1649307..1649516	-	210	WP_003527838.1	dodecin family protein	-
BN406_RS33800	1649806..1650252	+	447	WP_014527651.1	DNA-binding protein	-
BN406_RS33805	1650256..1650441	-	186	WP_010974989.1	hypothetical protein	-
BN406_RS33810	1650608..1651006	-	399	WP_010974988.1	hypothetical protein	-
BN406_RS36745	1651587..1651847	+	261	Protein_1507	helix-turn-helix domain containing protein	-
BN406_RS33815	1651928..1652224	-	297	WP_010974986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BN406_RS33820	1652214..1652462	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BN406_RS33830	1653080..1653205	+	126	Protein_1510	IS481 family transposase	-
BN406_RS33835	1653289..1654083	-	795	WP_013850206.1	methyltransferase domain-containing protein	-
BN406_RS33840	1654087..1654887	-	801	WP_015008735.1	ABC transporter ATP-binding protein	-
BN406_RS33845	1654884..1655927	-	1044	WP_010974980.1	iron ABC transporter permease	-
BN406_RS33850	1655924..1657009	-	1086	WP_015008736.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1664896	1664896
	-	flank	IS/Tn	-	-	1653014..1653205	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11473.15 Da
• Isoelectric Point: 10.6593

>T28406 WP_010974986.1 NC_018701:c1652224-1651928 [Sinorhizobium meliloti Rm41]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T28406 NC_018701:c1652224-1651928 [Sinorhizobium meliloti Rm41]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT28406 WP_003527850.1 NC_018701:c1652462-1652214 [Sinorhizobium meliloti Rm41]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT28406 NC_018701:c1652462-1652214 [Sinorhizobium meliloti Rm41]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: Q92X99

Antitoxin

• AlphaFold DB: H0FXK0