Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1406137..1406720 Replicon chromosome
Accession NZ_CP127297
Organism Escherichia coli strain APEC20/16

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag QRM67_RS06770 Protein ID WP_000254745.1
Coordinates 1406385..1406720 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QRM67_RS06765 Protein ID WP_000581937.1
Coordinates 1406137..1406385 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM67_RS06755 (1402476) 1402476..1403777 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QRM67_RS06760 (1403825) 1403825..1406059 + 2235 WP_000226815.1 GTP pyrophosphokinase -
QRM67_RS06765 (1406137) 1406137..1406385 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QRM67_RS06770 (1406385) 1406385..1406720 + 336 WP_000254745.1 endoribonuclease MazF Toxin
QRM67_RS06775 (1406791) 1406791..1407582 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
QRM67_RS06780 (1407810) 1407810..1409447 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QRM67_RS06785 (1409535) 1409535..1410833 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T284056 WP_000254745.1 NZ_CP127297:1406385-1406720 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT284056 WP_000581937.1 NZ_CP127297:1406137-1406385 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References