Detailed information of TA system TA028405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1299516..1300183
• Replicon: plasmid pSYMB
• Accession: NC_018701
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BN406_RS32155
• Protein ID: WP_015008546.1
• Coordinates: 1299740..1300183 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92WE1
• Locus tag: BN406_RS32150
• Protein ID: WP_010975287.1
• Coordinates: 1299516..1299743 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS32125	1294909..1295790	-	882	WP_003525869.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
BN406_RS32130	1295908..1297200	-	1293	WP_015008543.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
BN406_RS32140	1297570..1298859	+	1290	WP_015008544.1	SDR family oxidoreductase	-
BN406_RS32145	1298859..1299332	+	474	WP_015008545.1	DUF2269 family protein	-
BN406_RS32150	1299516..1299743	+	228	WP_010975287.1	hypothetical protein	Antitoxin
BN406_RS32155	1299740..1300183	+	444	WP_015008546.1	PIN domain-containing protein	Toxin
BN406_RS32160	1300199..1301137	-	939	WP_015008547.1	choline ABC transporter substrate-binding protein	-
BN406_RS32165	1301206..1302036	-	831	WP_013849981.1	SDR family oxidoreductase	-
BN406_RS32170	1302033..1302530	-	498	WP_015008548.1	acetone carboxylase subunit gamma	-
BN406_RS32175	1302534..1304726	-	2193	WP_015008549.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1664896	1664896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15649.96 Da
• Isoelectric Point: 6.9771

>T28405 WP_015008546.1 NC_018701:1299740-1300183 [Sinorhizobium meliloti Rm41]
VTFLLDVNVLIALIDPSHIGHNDAHEWFASIGQTTWATCPITENGVIRIVGNPEYPNSPGSPLPVMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Nucleotide

• Length: 444 bp

>T28405 NC_018701:1299740-1300183 [Sinorhizobium meliloti Rm41]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATAATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCACGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8174.39 Da
• Isoelectric Point: 9.1096

>AT28405 WP_010975287.1 NC_018701:1299516-1299743 [Sinorhizobium meliloti Rm41]
MRTTLAIDDDVLIAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Nucleotide

• Length: 228 bp

>AT28405 NC_018701:1299516-1299743 [Sinorhizobium meliloti Rm41]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCACCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q92WE1