Detailed information of TA system TA028404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 951363..952295
• Replicon: plasmid pSYMB
• Accession: NC_018701
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: -
• Uniprot ID: F7XGP1
• Locus tag: BN406_RS30555
• Protein ID: WP_014530511.1
• Coordinates: 951363..951917 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: F7XGP2
• Locus tag: BN406_RS30560
• Protein ID: WP_014530512.1
• Coordinates: 951924..952295 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS30535	946892..947758	+	867	WP_015008365.1	sulfate ABC transporter permease subunit CysT	-
BN406_RS30540	947748..948623	+	876	WP_013850796.1	sulfate ABC transporter permease subunit CysW	-
BN406_RS30545	948639..949745	+	1107	WP_015008366.1	sulfate/molybdate ABC transporter ATP-binding protein	-
BN406_RS30550	949857..951227	+	1371	WP_015008367.1	FAD-binding oxidoreductase	-
BN406_RS30555	951363..951917	-	555	WP_014530511.1	RES family NAD+ phosphorylase	Toxin
BN406_RS30560	951924..952295	-	372	WP_014530512.1	DUF2384 domain-containing protein	Antitoxin
BN406_RS30565	952372..953157	-	786	WP_014530513.1	crotonase/enoyl-CoA hydratase family protein	-
BN406_RS30570	953154..954023	-	870	WP_014530514.1	hydroxymethylglutaryl-CoA lyase	-
BN406_RS30575	954020..956008	-	1989	WP_015008368.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
BN406_RS30580	956025..956408	-	384	WP_010975573.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1664896	1664896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (23-177)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20449.31 Da
• Isoelectric Point: 6.4901

>T28404 WP_014530511.1 NC_018701:c951917-951363 [Sinorhizobium meliloti Rm41]
MRKHIPPASRPDSEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Nucleotide

• Length: 555 bp

>T28404 NC_018701:c951917-951363 [Sinorhizobium meliloti Rm41]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATTCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTCCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13117.25 Da
• Isoelectric Point: 9.6233

>AT28404 WP_014530512.1 NC_018701:c952295-951924 [Sinorhizobium meliloti Rm41]
MMAVDFQIAAARFGDDHSPFLSARLVADWLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT28404 NC_018701:c952295-951924 [Sinorhizobium meliloti Rm41]
ATGATGGCCGTTGATTTCCAGATAGCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACTGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Structures

Toxin

• AlphaFold DB: A0A222K2B4

Antitoxin

• AlphaFold DB: A0A222K374