Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3052228..3052907 Replicon chromosome
Accession NZ_CP127277
Organism Mycobacterium tuberculosis strain 25421

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag QRF07_RS14500 Protein ID WP_003414059.1
Coordinates 3052228..3052644 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag QRF07_RS14505 Protein ID WP_003414061.1
Coordinates 3052641..3052907 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF07_RS14480 (QRF07_14480) 3048280..3049182 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
QRF07_RS14485 (QRF07_14485) 3049251..3050003 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
QRF07_RS14490 (QRF07_14490) 3050247..3050522 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
QRF07_RS14495 (QRF07_14495) 3050519..3052141 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
QRF07_RS14500 (QRF07_14500) 3052228..3052644 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
QRF07_RS14505 (QRF07_14505) 3052641..3052907 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QRF07_RS14510 (QRF07_14510) 3052933..3053328 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
QRF07_RS14515 (QRF07_14515) 3053325..3053594 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
QRF07_RS14520 (QRF07_14520) 3053604..3054698 - 1095 WP_003414068.1 restriction endonuclease subunit S -
QRF07_RS14525 (QRF07_14525) 3054695..3055114 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
QRF07_RS14530 (QRF07_14530) 3055113..3055187 + 75 Protein_2867 hypothetical protein -
QRF07_RS14535 (QRF07_14535) 3055188..3055667 - 480 WP_003414073.1 dihydrofolate reductase -
QRF07_RS14540 (QRF07_14540) 3055738..3056538 - 801 WP_003911953.1 thymidylate synthase -
QRF07_RS14545 (QRF07_14545) 3056694..3057431 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T284038 WP_003414059.1 NZ_CP127277:c3052644-3052228 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT284038 WP_003414061.1 NZ_CP127277:c3052907-3052641 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References