Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2851785..2852422 Replicon chromosome
Accession NZ_CP127277
Organism Mycobacterium tuberculosis strain 25421

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag QRF07_RS13415 Protein ID WP_003413180.1
Coordinates 2851785..2852180 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag QRF07_RS13420 Protein ID WP_003413183.1
Coordinates 2852177..2852422 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF07_RS13375 (QRF07_13375) 2847188..2848399 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
QRF07_RS13380 (QRF07_13380) 2848526..2849185 + 660 WP_285978957.1 LppA family lipoprotein -
QRF07_RS13385 (QRF07_13385) 2849182..2849844 + 663 WP_286027199.1 LppA family lipoprotein -
QRF07_RS13390 (QRF07_13390) 2849841..2850119 + 279 WP_041018915.1 type II toxin-antitoxin system VapB family antitoxin -
QRF07_RS13395 (QRF07_13395) 2850212..2850625 + 414 WP_003413164.1 PIN domain nuclease -
QRF07_RS13400 (QRF07_13400) 2850664..2850921 + 258 WP_003413167.1 CopG family transcriptional regulator -
QRF07_RS13405 (QRF07_13405) 2850918..2851295 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
QRF07_RS13410 (QRF07_13410) 2851311..2851685 - 375 WP_003413177.1 hypothetical protein -
QRF07_RS13415 (QRF07_13415) 2851785..2852180 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
QRF07_RS13420 (QRF07_13420) 2852177..2852422 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
QRF07_RS13425 (QRF07_13425) 2852833..2853252 - 420 WP_003413190.1 A24 family peptidase -
QRF07_RS13430 (QRF07_13430) 2853264..2854073 - 810 WP_003413193.1 shikimate dehydrogenase -
QRF07_RS13435 (QRF07_13435) 2854070..2855323 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
QRF07_RS13440 (QRF07_13440) 2855316..2855828 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T284035 WP_003413180.1 NZ_CP127277:c2852180-2851785 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT284035 WP_003413183.1 NZ_CP127277:c2852422-2852177 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References