Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2216926..2217512 Replicon chromosome
Accession NZ_CP127277
Organism Mycobacterium tuberculosis strain 25421

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag QRF07_RS10405 Protein ID WP_003410010.1
Coordinates 2216926..2217270 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag QRF07_RS10410 Protein ID WP_003410014.1
Coordinates 2217264..2217512 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF07_RS10370 (QRF07_10370) 2212632..2213231 + 600 WP_003910883.1 L-lysine exporter -
QRF07_RS10375 (QRF07_10375) 2213647..2214075 + 429 WP_003409992.1 cellulose-binding protein -
QRF07_RS10380 (QRF07_10380) 2214301..2214840 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
QRF07_RS10385 (QRF07_10385) 2215360..2215920 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
QRF07_RS10390 (QRF07_10390) 2215917..2216258 - 342 WP_003410003.1 DUF2384 domain-containing protein -
QRF07_RS10395 (QRF07_10395) 2216344..2216601 + 258 WP_003410006.1 hypothetical protein -
QRF07_RS10400 (QRF07_10400) 2216502..2216837 - 336 WP_003410009.1 dehydrogenase -
QRF07_RS10405 (QRF07_10405) 2216926..2217270 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
QRF07_RS10410 (QRF07_10410) 2217264..2217512 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
QRF07_RS10415 (QRF07_10415) 2217612..2219927 - 2316 WP_003899120.1 cation transporter ATPase CptG -
QRF07_RS10420 (QRF07_10420) 2219924..2220196 - 273 WP_003410017.1 DUF1490 family protein -
QRF07_RS10425 (QRF07_10425) 2220249..2220605 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
QRF07_RS10430 (QRF07_10430) 2220762..2221529 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T284025 WP_003410010.1 NZ_CP127277:c2217270-2216926 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT284025 WP_003410014.1 NZ_CP127277:c2217512-2217264 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References