Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1685906..1686522 Replicon chromosome
Accession NZ_CP127277
Organism Mycobacterium tuberculosis strain 25421

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag QRF07_RS07950 Protein ID WP_003407593.1
Coordinates 1686205..1686522 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag QRF07_RS07945 Protein ID WP_003900349.1
Coordinates 1685906..1686208 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF07_RS07935 (QRF07_07935) 1681792..1683639 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
QRF07_RS07940 (QRF07_07940) 1683640..1685892 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
QRF07_RS07945 (QRF07_07945) 1685906..1686208 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
QRF07_RS07950 (QRF07_07950) 1686205..1686522 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
QRF07_RS07955 (QRF07_07955) 1686519..1687523 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
QRF07_RS07960 (QRF07_07960) 1687576..1688865 + 1290 WP_003902090.1 serine hydrolase domain-containing protein -
QRF07_RS07965 (QRF07_07965) 1688938..1689663 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
QRF07_RS07970 (QRF07_07970) 1689769..1689981 - 213 WP_003898900.1 dodecin family protein -
QRF07_RS07975 (QRF07_07975) 1690042..1690440 + 399 WP_003900351.1 hypothetical protein -
QRF07_RS07980 (QRF07_07980) 1690485..1691513 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T284016 WP_003407593.1 NZ_CP127277:1686205-1686522 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT284016 WP_003900349.1 NZ_CP127277:1685906-1686208 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References