Detailed information of TA system TA028401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2561812..2562578
• Replicon: chromosome
• Accession: NC_018700
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: BN406_RS12580
• Protein ID: WP_003533488.1
• Coordinates: 2562096..2562578 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: BN406_RS12575
• Protein ID: WP_003533490.1
• Coordinates: 2561812..2562096 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS12560	2557282..2558670	+	1389	WP_015007940.1	lipopolysaccharide biosynthesis protein	-
BN406_RS12565	2558674..2559945	+	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
BN406_RS12570	2559961..2561607	-	1647	WP_010970088.1	AMP-binding protein	-
BN406_RS12575	2561812..2562096	+	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
BN406_RS12580	2562096..2562578	+	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
BN406_RS12590	2563043..2563552	+	510	WP_013844819.1	disulfide bond formation protein B	-
BN406_RS12595	2563584..2564141	-	558	WP_003533486.1	HNH endonuclease	-
BN406_RS12600	2564247..2564894	-	648	WP_010970091.1	DNA-3-methyladenine glycosylase	-
BN406_RS12605	2565047..2565943	+	897	WP_015007941.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
BN406_RS12610	2565962..2566837	-	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T28401 WP_003533488.1 NC_018700:2562096-2562578 [Sinorhizobium meliloti Rm41]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T28401 NC_018700:2562096-2562578 [Sinorhizobium meliloti Rm41]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT28401 WP_003533490.1 NC_018700:2561812-2562096 [Sinorhizobium meliloti Rm41]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT28401 NC_018700:2561812-2562096 [Sinorhizobium meliloti Rm41]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4