Detailed information of TA system TA028399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1832925..1833579
• Replicon: chromosome
• Accession: NC_018700
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: BN406_RS08930
• Protein ID: WP_003530010.1
• Coordinates: 1832925..1833113 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: BN406_RS08935
• Protein ID: WP_010969659.1
• Coordinates: 1833118..1833579 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS08915	1829491..1830801	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
BN406_RS08920	1830826..1831797	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
BN406_RS08925	1831978..1832748	-	771	WP_010969658.1	hypothetical protein	-
BN406_RS08930	1832925..1833113	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
BN406_RS08935	1833118..1833579	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BN406_RS08945	1834438..1834644	+	207	WP_015007715.1	hypothetical protein	-
BN406_RS08950	1834899..1835171	+	273	WP_015007716.1	hypothetical protein	-
BN406_RS34595	1835182..1835490	-	309	WP_015007717.1	hypothetical protein	-
BN406_RS08955	1835602..1836576	-	975	WP_041169323.1	non-homologous end-joining DNA ligase	-
BN406_RS37985	1836669..1836839	+	171	WP_015007719.1	hypothetical protein	-
BN406_RS33995	1837344..1837517	-	174	WP_015007721.1	DUF3606 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T28399 WP_003530010.1 NC_018700:1832925-1833113 [Sinorhizobium meliloti Rm41]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T28399 NC_018700:1832925-1833113 [Sinorhizobium meliloti Rm41]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT28399 WP_010969659.1 NC_018700:1833118-1833579 [Sinorhizobium meliloti Rm41]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT28399 NC_018700:1833118-1833579 [Sinorhizobium meliloti Rm41]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0