Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3094975..3095545 Replicon chromosome
Accession NZ_CP127276
Organism Mycobacterium tuberculosis strain 5521

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag QRF08_RS14770 Protein ID WP_003414166.1
Coordinates 3094975..3095331 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag QRF08_RS14775 Protein ID WP_003901465.1
Coordinates 3095315..3095545 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF08_RS14750 (QRF08_14750) 3090427..3092115 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
QRF08_RS14755 (QRF08_14755) 3092119..3092445 - 327 WP_003414157.1 hypothetical protein -
QRF08_RS14760 (QRF08_14760) 3092618..3093205 + 588 WP_003914429.1 DUF3558 family protein -
QRF08_RS14765 (QRF08_14765) 3093224..3094873 + 1650 Protein_2914 CocE/NonD family hydrolase -
QRF08_RS14770 (QRF08_14770) 3094975..3095331 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
QRF08_RS14775 (QRF08_14775) 3095315..3095545 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
QRF08_RS14780 (QRF08_14780) 3095588..3096631 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
QRF08_RS14785 (QRF08_14785) 3096630..3097097 + 468 WP_003414177.1 DUF1778 domain-containing protein -
QRF08_RS14790 (QRF08_14790) 3097273..3097527 - 255 WP_003917684.1 hypothetical protein -
QRF08_RS14795 (QRF08_14795) 3097675..3098079 + 405 WP_003414181.1 hypothetical protein -
QRF08_RS14800 (QRF08_14800) 3098076..3098267 + 192 WP_003414184.1 hypothetical protein -
QRF08_RS14805 (QRF08_14805) 3098484..3098744 + 261 Protein_2922 transposase -
QRF08_RS14810 (QRF08_14810) 3099854..3100111 + 258 WP_003899489.1 hypothetical protein -
QRF08_RS14815 (QRF08_14815) 3100216..3100527 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T283979 WP_003414166.1 NZ_CP127276:c3095331-3094975 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT283979 WP_003901465.1 NZ_CP127276:c3095545-3095315 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References