Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3054978..3055657 Replicon chromosome
Accession NZ_CP127276
Organism Mycobacterium tuberculosis strain 5521

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag QRF08_RS14540 Protein ID WP_003414059.1
Coordinates 3054978..3055394 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag QRF08_RS14545 Protein ID WP_003414061.1
Coordinates 3055391..3055657 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF08_RS14520 (QRF08_14520) 3051030..3051932 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
QRF08_RS14525 (QRF08_14525) 3052001..3052753 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
QRF08_RS14530 (QRF08_14530) 3052997..3053272 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
QRF08_RS14535 (QRF08_14535) 3053269..3054891 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
QRF08_RS14540 (QRF08_14540) 3054978..3055394 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
QRF08_RS14545 (QRF08_14545) 3055391..3055657 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QRF08_RS14550 (QRF08_14550) 3055683..3056078 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
QRF08_RS14555 (QRF08_14555) 3056075..3056344 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
QRF08_RS14560 (QRF08_14560) 3056354..3057448 - 1095 WP_003414068.1 restriction endonuclease subunit S -
QRF08_RS14565 (QRF08_14565) 3057445..3057864 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
QRF08_RS14570 (QRF08_14570) 3057863..3057937 + 75 Protein_2875 hypothetical protein -
QRF08_RS14575 (QRF08_14575) 3057938..3058417 - 480 WP_003414073.1 dihydrofolate reductase -
QRF08_RS14580 (QRF08_14580) 3058488..3059288 - 801 WP_003911953.1 thymidylate synthase -
QRF08_RS14585 (QRF08_14585) 3059444..3060181 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T283977 WP_003414059.1 NZ_CP127276:c3055394-3054978 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT283977 WP_003414061.1 NZ_CP127276:c3055657-3055391 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References