Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2219137..2219723 Replicon chromosome
Accession NZ_CP127276
Organism Mycobacterium tuberculosis strain 5521

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag QRF08_RS10445 Protein ID WP_003410010.1
Coordinates 2219137..2219481 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag QRF08_RS10450 Protein ID WP_003410014.1
Coordinates 2219475..2219723 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF08_RS10410 (QRF08_10410) 2214843..2215442 + 600 WP_003910883.1 L-lysine exporter -
QRF08_RS10415 (QRF08_10415) 2215858..2216286 + 429 WP_003409992.1 cellulose-binding protein -
QRF08_RS10420 (QRF08_10420) 2216512..2217051 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
QRF08_RS10425 (QRF08_10425) 2217571..2218131 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
QRF08_RS10430 (QRF08_10430) 2218128..2218469 - 342 WP_003410003.1 DUF2384 domain-containing protein -
QRF08_RS10435 (QRF08_10435) 2218555..2218812 + 258 WP_003410006.1 hypothetical protein -
QRF08_RS10440 (QRF08_10440) 2218713..2219048 - 336 WP_003410009.1 dehydrogenase -
QRF08_RS10445 (QRF08_10445) 2219137..2219481 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
QRF08_RS10450 (QRF08_10450) 2219475..2219723 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
QRF08_RS10455 (QRF08_10455) 2219823..2222138 - 2316 WP_003899120.1 cation transporter ATPase CptG -
QRF08_RS10460 (QRF08_10460) 2222135..2222407 - 273 WP_003410017.1 DUF1490 family protein -
QRF08_RS10465 (QRF08_10465) 2222460..2222816 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
QRF08_RS10470 (QRF08_10470) 2222973..2223740 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T283964 WP_003410010.1 NZ_CP127276:c2219481-2219137 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT283964 WP_003410014.1 NZ_CP127276:c2219723-2219475 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References