Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1685493..1686109 Replicon chromosome
Accession NZ_CP127276
Organism Mycobacterium tuberculosis strain 5521

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag QRF08_RS07955 Protein ID WP_003407593.1
Coordinates 1685792..1686109 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag QRF08_RS07950 Protein ID WP_003900349.1
Coordinates 1685493..1685795 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF08_RS07940 (QRF08_07940) 1681379..1683226 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
QRF08_RS07945 (QRF08_07945) 1683227..1685479 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
QRF08_RS07950 (QRF08_07950) 1685493..1685795 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
QRF08_RS07955 (QRF08_07955) 1685792..1686109 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
QRF08_RS07960 (QRF08_07960) 1686106..1687110 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
QRF08_RS07965 (QRF08_07965) 1687163..1688452 + 1290 WP_003902090.1 serine hydrolase domain-containing protein -
QRF08_RS07970 (QRF08_07970) 1688525..1689250 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
QRF08_RS07975 (QRF08_07975) 1689356..1689568 - 213 WP_003898900.1 dodecin family protein -
QRF08_RS07980 (QRF08_07980) 1689629..1690027 + 399 WP_003900351.1 hypothetical protein -
QRF08_RS07985 (QRF08_07985) 1690072..1691100 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T283955 WP_003407593.1 NZ_CP127276:1685792-1686109 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT283955 WP_003900349.1 NZ_CP127276:1685493-1685795 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References