Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1229882..1230513 Replicon chromosome
Accession NZ_CP127276
Organism Mycobacterium tuberculosis strain 5521

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag QRF08_RS05900 Protein ID WP_003405820.1
Coordinates 1229882..1230193 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag QRF08_RS05905 Protein ID WP_003405836.1
Coordinates 1230193..1230513 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF08_RS05880 (QRF08_05880) 1225363..1226787 - 1425 WP_003405805.1 class II fumarate hydratase -
QRF08_RS05885 (QRF08_05885) 1226818..1227906 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
QRF08_RS05890 (QRF08_05890) 1227962..1228606 + 645 WP_003915494.1 DUF4245 domain-containing protein -
QRF08_RS05895 (QRF08_05895) 1228613..1229770 - 1158 WP_003898727.1 AI-2E family transporter -
QRF08_RS05900 (QRF08_05900) 1229882..1230193 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
QRF08_RS05905 (QRF08_05905) 1230193..1230513 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
QRF08_RS05910 (QRF08_05910) 1230523..1232059 + 1537 Protein_1164 carboxylesterase/lipase family protein -
QRF08_RS05915 (QRF08_05915) 1232066..1233178 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
QRF08_RS05920 (QRF08_05920) 1233188..1233445 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
QRF08_RS05925 (QRF08_05925) 1233435..1234682 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
QRF08_RS05930 (QRF08_05930) 1234679..1235317 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T283949 WP_003405820.1 NZ_CP127276:c1230193-1229882 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT283949 WP_003405836.1 NZ_CP127276:c1230513-1230193 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References