Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 142038..142603 Replicon plasmid pSYMBR3459
Accession NC_018696
Organism Burkholderia phenoliruptrix BR3459a

Toxin (Protein)


Gene name vapC Uniprot ID K0DYP8
Locus tag BUPH_RS30735 Protein ID WP_015004268.1
Coordinates 142038..142409 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6J5KG26
Locus tag BUPH_RS30740 Protein ID WP_041746923.1
Coordinates 142406..142603 (-) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BUPH_RS30710 137846..139267 + 1422 WP_015004263.1 ISNCY family transposase -
BUPH_RS30715 139506..139901 - 396 WP_015004264.1 DUF3564 domain-containing protein -
BUPH_RS30720 140112..140699 + 588 WP_015004265.1 hypothetical protein -
BUPH_RS30725 140943..141383 + 441 WP_015004266.1 DUF2384 domain-containing protein -
BUPH_RS30730 141383..141856 + 474 WP_015004267.1 RES family NAD+ phosphorylase -
BUPH_RS30735 142038..142409 - 372 WP_015004268.1 type II toxin-antitoxin system VapC family toxin Toxin
BUPH_RS30740 142406..142603 - 198 WP_041746923.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BUPH_RS30745 142864..143508 - 645 WP_015004269.1 hypothetical protein -
BUPH_RS30750 143648..145252 - 1605 WP_015004270.1 sensor domain-containing diguanylate cyclase -
BUPH_RS30755 145661..145852 + 192 WP_041746924.1 hypothetical protein -
BUPH_RS30760 146548..147507 + 960 WP_148281872.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..785419 785419


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 14007.32 Da        Isoelectric Point: 5.5836

>T28394 WP_015004268.1 NC_018696:c142409-142038 [Paraburkholderia phenoliruptrix BR3459a]
MILVDTSVWIDHIDRSEPIMVDLLLHDRVRIHPYVIGEISLGSLRDRSIVLRALNDLPRVPVATPDEVFYLIEQQQLFSR
GIGYVDASLLASARLEPGVMIWTRDKRLKKIADDLALSPVLEH

Download         Length: 372 bp

>T28394 NC_018696:c142409-142038 [Paraburkholderia phenoliruptrix BR3459a]
ATGATCCTCGTCGACACGTCCGTGTGGATCGATCATATTGACCGGTCAGAGCCGATAATGGTCGACCTTCTGCTACATGA
CCGTGTCCGAATCCATCCATACGTGATCGGCGAAATTTCGCTAGGCAGTTTGCGCGACCGTTCTATCGTGCTTCGTGCTT
TGAACGACCTGCCTCGCGTGCCCGTTGCGACGCCGGACGAAGTCTTCTATCTGATCGAGCAGCAACAATTATTCAGCCGG
GGAATTGGCTACGTAGACGCATCCTTGCTCGCATCTGCCAGGCTGGAGCCTGGTGTGATGATCTGGACGCGGGACAAGAG
ATTGAAAAAAATCGCCGACGACCTTGCGCTAAGCCCGGTTCTGGAGCACTAG

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7106.05 Da        Isoelectric Point: 8.7162

>AT28394 WP_041746923.1 NC_018696:c142603-142406 [Paraburkholderia phenoliruptrix BR3459a]
MRTTIALDDDLVSRAQDYTGLNEKSALVREALKALIEREAARRLAALGGSQPGITGAPRRRQEVE

Download         Length: 198 bp

>AT28394 NC_018696:c142603-142406 [Paraburkholderia phenoliruptrix BR3459a]
ATGCGAACCACAATTGCTTTGGACGATGACCTGGTCTCCCGGGCCCAAGACTACACGGGGTTGAACGAGAAATCCGCTCT
TGTGCGGGAGGCATTGAAGGCGTTAATCGAACGTGAAGCTGCGCGCCGGCTCGCCGCCTTGGGGGGCAGTCAACCGGGCA
TCACTGGCGCGCCGCGTCGGCGGCAGGAGGTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K0DYP8

Antitoxin

Source ID Structure
AlphaFold DB A0A6J5KG26

References