Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 749501..750134 Replicon chromosome
Accession NZ_CP127275
Organism Mycobacterium tuberculosis strain 6721

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag QRF09_RS03450 Protein ID WP_003403365.1
Coordinates 749501..749884 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag QRF09_RS03455 Protein ID WP_003403368.1
Coordinates 749979..750134 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF09_RS03425 (QRF09_03425) 744793..745329 + 537 WP_003403341.1 50S ribosomal protein L10 -
QRF09_RS03430 (QRF09_03430) 745366..745758 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
QRF09_RS03435 (QRF09_03435) 745751..746446 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
QRF09_RS03440 (QRF09_03440) 746517..748022 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
QRF09_RS03445 (QRF09_03445) 748103..749113 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
QRF09_RS03450 (QRF09_03450) 749501..749884 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
QRF09_RS03455 (QRF09_03455) 749979..750134 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QRF09_RS03460 (QRF09_03460) 750210..750926 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
QRF09_RS03465 (QRF09_03465) 751202..751510 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
QRF09_RS03470 (QRF09_03470) 751497..751742 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
QRF09_RS03475 (QRF09_03475) 751852..752289 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
QRF09_RS03480 (QRF09_03480) 752286..752540 - 255 WP_003911263.1 antitoxin VapB7 -
QRF09_RS03485 (QRF09_03485) 752654..755017 + 2364 WP_003901895.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T283880 WP_003403365.1 NZ_CP127275:c749884-749501 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T283880 NZ_CP127275:c749884-749501 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT283880 WP_003403368.1 NZ_CP127275:c750134-749979 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT283880 NZ_CP127275:c750134-749979 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References