Detailed information of TA system TA028383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 221907..222493
• Replicon: chromosome
• Accession: NC_018691
• Organism: Alcanivorax dieselolei B5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B5T_RS01050
• Protein ID: WP_041716636.1
• Coordinates: 222113..222493 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K0C7H7
• Locus tag: B5T_RS01045
• Protein ID: WP_014992579.1
• Coordinates: 221907..222116 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5T_RS01025	217812..218999	+	1188	WP_014992575.1	CoA transferase	-
B5T_RS01030	219020..220018	+	999	WP_014992576.1	NADPH:quinone oxidoreductase family protein	-
B5T_RS01035	220028..220528	+	501	WP_014992577.1	TRAP transporter small permease	-
B5T_RS01040	220525..221832	+	1308	WP_014992578.1	TRAP transporter large permease subunit	-
B5T_RS01045	221907..222116	+	210	WP_014992579.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B5T_RS01050	222113..222493	+	381	WP_041716636.1	type II toxin-antitoxin system VapC family toxin	Toxin
B5T_RS01055	222651..223838	-	1188	WP_041717209.1	fatty acid desaturase	-
B5T_RS01060	223926..225935	-	2010	WP_041716638.1	hydantoinase/oxoprolinase family protein	-
B5T_RS01065	226189..226998	+	810	WP_014992583.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14271.59 Da
• Isoelectric Point: 6.4827

>T28383 WP_041716636.1 NC_018691:222113-222493 [Alcanivorax dieselolei B5]
MSVLVDTSVWVAHFREKNEALINLIEMDRALVHPMVLGELACGTPPEPRQRTLGYIGLLRLSNQASLKEVMTFVEQERLY
GRGRGLVDMMLLASTVITPEGRLWTLDKRLAGLADEFDVAYQPHRH

Nucleotide

• Length: 381 bp

>T28383 NC_018691:222113-222493 [Alcanivorax dieselolei B5]
ATGAGCGTTTTGGTGGATACCTCGGTATGGGTGGCGCACTTCAGAGAAAAAAATGAGGCGCTGATTAACCTGATAGAAAT
GGACCGCGCCCTGGTGCATCCCATGGTCTTGGGCGAGCTTGCCTGCGGGACTCCGCCTGAACCTCGGCAACGAACTTTGG
GTTACATTGGTTTATTGCGGCTGAGTAATCAGGCCAGCCTTAAGGAGGTCATGACGTTCGTTGAGCAAGAACGCTTATAC
GGGCGAGGTCGTGGTTTGGTGGATATGATGTTGCTCGCTTCAACCGTCATCACTCCCGAAGGGAGGCTTTGGACGTTGGA
TAAACGCTTGGCTGGATTGGCGGATGAGTTCGACGTGGCGTACCAGCCGCATAGGCATTAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7666.71 Da
• Isoelectric Point: 4.5557

>AT28383 WP_014992579.1 NC_018691:221907-222116 [Alcanivorax dieselolei B5]
MRTTITIDDALYEQALELADSGLDKPADILREAVKTYVRVQAARRLAALGGASPEMADIPRRREEPLQE

Nucleotide

• Length: 210 bp

>AT28383 NC_018691:221907-222116 [Alcanivorax dieselolei B5]
ATGCGTACCACAATTACGATTGACGATGCCCTCTACGAGCAGGCATTGGAGCTGGCTGACTCAGGCTTGGATAAACCCGC
GGATATTCTTCGGGAGGCCGTCAAAACCTATGTTCGGGTCCAAGCCGCGCGCCGCCTGGCCGCTTTGGGAGGAGCGTCAC
CTGAGATGGCGGATATCCCGCGGCGGCGTGAGGAGCCGTTGCAGGAATGA

Structures

Antitoxin

• AlphaFold DB: K0C7H7