Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 1024210..1025183 | Replicon | chromosome |
| Accession | NZ_CP127274 | ||
| Organism | Mycobacterium tuberculosis strain 17221 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | O05911 |
| Locus tag | QRF10_RS04885 | Protein ID | WP_003404756.1 |
| Coordinates | 1024683..1025183 (+) | Length | 167 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | O05910 |
| Locus tag | QRF10_RS04880 | Protein ID | WP_003898647.1 |
| Coordinates | 1024210..1024686 (+) | Length | 159 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QRF10_RS04860 (QRF10_04860) | 1020057..1021328 | - | 1272 | WP_003404747.1 | PPE family protein | - |
| QRF10_RS04865 (QRF10_04865) | 1021343..1021642 | - | 300 | WP_003404748.1 | PE family protein | - |
| QRF10_RS04870 (QRF10_04870) | 1022086..1023867 | + | 1782 | WP_003404749.1 | BCCT family transporter | - |
| QRF10_RS04875 (QRF10_04875) | 1023867..1024121 | + | 255 | WP_003898646.1 | hypothetical protein | - |
| QRF10_RS04880 (QRF10_04880) | 1024210..1024686 | + | 477 | WP_003898647.1 | DUF1778 domain-containing protein | Antitoxin |
| QRF10_RS04885 (QRF10_04885) | 1024683..1025183 | + | 501 | WP_003404756.1 | GNAT family N-acetyltransferase | Toxin |
| QRF10_RS04895 (QRF10_04895) | 1025496..1026815 | - | 1320 | WP_003404757.1 | IS256-like element IS1554 family transposase | - |
| QRF10_RS04900 (QRF10_04900) | 1027103..1027684 | + | 582 | WP_003404760.1 | IS607-like element IS1535 family transposase | - |
| QRF10_RS04905 (QRF10_04905) | 1027684..1029336 | + | 1653 | WP_003404761.1 | IS607 family element RNA-guided endonuclease TnpB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1023864..1069775 | 45911 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 167 a.a. Molecular weight: 18048.55 Da Isoelectric Point: 7.8659
>T283826 WP_003404756.1 NZ_CP127274:1024683-1025183 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD
Download Length: 501 bp
Antitoxin
Download Length: 159 a.a. Molecular weight: 17387.98 Da Isoelectric Point: 11.2467
>AT283826 WP_003898647.1 NZ_CP127274:1024210-1024686 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG
Download Length: 477 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U4BSZ2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A5U0V8 |